This study was designed to determine the effect of different osmotic pressures on spermatozoa characteristics for cryopreservation of buffalo bull semen using tris egg yolk extender (TEYE). Semen of Nili-Ravi buffalo bulls (n = 04) housed at Semen Production Unit, Qadirabad, Sahiwal, Pakistan, was collected at weekly interval for 10 weeks. Three solutions of tris-citric acid-fructose with osmot...

the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg ml-1 sodium selenite and the sperm motility and...

Multiple DNA-associated processes such as DNA repair, replication, and recombination are crucial for the maintenance of genome integrity. Here, we show a novel interaction between the transcription elongation factor Bur1-Bur2 and replication protein A (RPA), the eukaryotic single-stranded DNA-binding protein with functions in DNA repair, recombination, and replication. Bur1 interacted via its C...

In this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (GSH) on buffalo (Bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and DNA integrity as well as in vivo fertility. Semen from three Nili-Ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (...

The protein kinase C (PKC) superfamily plays key regulatory roles in numerous cellular processes. Saccharomyces cerevisiae contains a single PKC, Pkc1, whose main function is cell wall integrity maintenance. In this work, we connect the Pkc1 protein to the maintenance of genome integrity in response to genotoxic stresses. Pkc1 and its kinase activity are necessary for the phosphorylation of che...

BACKGROUND Occupational exposure to toxic agents may cause infertility, congenital anomalies or death in offspring, but few studies have evaluated DNA integrity in germ cells of male workers. We investigated sperm DNA integrity in individuals occupationally exposed to styrene. METHODS AND RESULTS Semen samples were obtained from 46 male workers exposed to styrene and 27 unexposed controls (ag...

BACKGROUND There are few studies indicating the detrimental effects of ibuprofen on sperm fertility potential and DNA integrity. OBJECTIVE To determine the effects of Ibuprofen on sperm parameters, chromatin condensation and DNA integrity of mice. MATERIALS AND METHODS In this experimental study, 36 adult male mice with average weight 37 gr were divided into three groups, including control ...

BACKGROUND The influence of ejaculatory abstinence (EA) on semen parameters and subsequent reproductive outcome is still debatable; hence understanding the impact of EA on sperm structural and functional integrity may provide a valuable information on predicting successful clinical outcome. OBJECTIVE To understand the influence of EA on sperm chromatin maturity, integrity, longevity and globa...

A network governing DNA integrity was identified in yeast by a global genetic analysis of synthetic fitness or lethality defect (SFL) interactions. Within this network, 16 functional modules or minipathways were defined based on patterns of global SFL interactions. Modules or genes involved in DNA replication, DNA-replication checkpoint (DRC) signaling, and oxidative stress response were identi...

The aim of our study was to look for alternative predictive biomarkers for breast cancer management in limited resource setup. A comprehensive analysis of circulating cell-free DNA (CCFD) in serum at baseline was performed to assess its prognostic potential. Quantitative polymerase chain reaction (qPCR) of ALU sequences using ALU115 and ALU247 primers was carried out in patients (N: baseline 14...