A theoretical mechanism is presented for the hologram of gravity. This principle turns the Big Bang-universe into an illusion. The keystone is described as refined time, which is combined with the relativistic time of General Relativity. The refined time is time smaller than the Plancktime, which is formulated in a set of formulas in the Double Torus Theory (DTT). This is a new cosmological the...

Intact yeast cells subjected to a punctual electric field (in an electroporator) can be transformed with exogenous DNA (1), indicating that DNA molecules can move into the cell through the cell wall. In pulsed-field electrophoresis, we have also observed that removal of the cell wall is an unnecessary step for the large chromosomal DNA molecules (ranging from 2000 to 240 Kb), as well as for mit...

We have synthesized a new reagent-N,N'-dimethyl-N,.,V.bis(mercaptoacetyl)hydrazine(DMH)-for the reduction of disulfide bonds in proieins. DMH reduce.s disulfide bonds ? times faster than does dithiothreitol (DTT) in water at pH ?. DMH reduces mixed disulfides of cvsteine proteases (papain and ficin) especially rapdily i30 ti-.r faster than DTT). DMH (eo = -0.300 V) reduces noncl'clic disulfides...

The rate constant for strand breakage in poly(U) after reaction with hydrogen atoms in deoxygenated aqueous solution has been determined to be k = 1.5 s-1 at pH = 4-5 and 24 degrees C. Dithiothreitol has been found to prevent strand break formation by reacting with H-adduct radicals of poly(U) with a rate constant of 5 X 10(6) M-1 s-1. It is concluded that the rate-determining step in H atom-in...

Nitrogen-doped, PEGylated carbon dots (C-dots) have been synthesized for the detection of mercury ions (Hg(2+)). The detection limit was found to be 6.8 nM. However, upon functionalization with dithiothreitol (DTT), it reached to as low as 18 pM. The C-dots-Hg(2+) system was also able to efficiently detect biothiols.

Disulfide- and terminal alkyne-modified magnetic silica particles (DA-MSPs) were synthesized and used to covalently capture and reductively release azido glycopeptides via click chemistry and dithiothreitol treatment. Using DA-MSPs, an efficient and specific enrichment method for separating azido glycopeptides has been developed.

A novel bis(ferrocenyl chalcone) silicon(IV) phthalocyanine has been prepared in which the disulfide linker can be cleaved by dithiothreitol. The separation of the ferrocenyl quencher and the phthalocyanine core greatly enhances the fluorescence emission, singlet oxygen production and in vitro photocytotoxicity.