The effects of plant growth regulators were examined in order to optimize the callus induction, regeneration, and proliferation of lisianthus (Eustoma grandiflorum). In vitro leaves provided the explants for callus induction. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of indole-3-acetic acid (IAA), 1-naphthaleneacetic acid (NAA), and 2,4-dichlorophen...

Establishment of a tissue culture protocol for clonal regeneration is an essential prerequisite the potential and economically important plant species Biancaea sappan (L.) Tod. (syn. Caesalpinia L.; Caesalpiniaceae). This valuable dye-yielding legume shrub native to Indomalayan used in India by local people Kerala Tamil Nadu States its biological potential. The present study evaluated seed deve...

Regeneration and gene expression of salt/drought stress tolerant Alhagi graecrum and stress sensitive Lycopersicon esculentum under different concentrations of NaCl and mannitol were studied. Mannitol and NaCl applied at concentrations of 86, 172, 256 and 43, 86, 172 mM, respectively, in a shoot regeneration medium, caused similar water potentials. In L. esculentum, regeneration was influenced ...

The effect of aqueous smoke extract, derived from Tanacetum parthenium, onin vitro plantlet regeneration from rapeseed (Brassica napus L. cv. Topas)microspore-derived embryos (MDEs) was evaluated in this study. Inoculation ofrapeseed MDEs with the smoke-extract dilutions (1:250, 1:500, 1:1000 and 1:2000)for various times (5, 10 and 15 min) significantly enhanced the percentage ofregenerated pla...

A reliable method is described for rapid proliferation of axillary shoots from nodal segment of mature field-grown aromatic and medicinal plant Vitex negundo through in vitro culture. Of different concentrations of N6-benzyladenine (BA) evaluated as supplements to Murahige and Skoog (MS) medium, BA at 1mg/l was effective in enhancing bud break and shoot regeneration. Response of explants was al...

Soybean, a major legume crop, is the source of vegetable oil and protein. There is a need for transgenic approaches to breeding superior soybean varieties to meet future climate challenges. Efficient plant regeneration is a prerequisite for successful application of genetic transformation technology. Soybean cultivars are classified into different maturity groups based on photoperiod requiremen...

This study was carried out to determine in vitro development using Black cumin leaf and stem explants. ?ameli black variety used as plant material. Five different nutrient mediums (1. LS2.5, 2. MS, 3. MS + 0.5 mg.l-1 IAA, 4. BAP, 5. IAA BAP) containing 30 g sugar were this study. As a result of the research, 100% callus formation detected explants cultured number 1 5 mediums. These followed by ...

Populus ussuriensis is an important tree species with high economic and ecologic values. However, traditional sexual propagation time-consuming inefficient, challenging afforestation wood production using P. ussuriensis, requires a rapid efficient regeneration system. The present study established rapid, efficient, stable shoot method from root explants in several plant growth regulators. Most ...