A novel protocol for production of multiple shoot tip clumps of Keelar nelli (Phyllanthus amarus Schum. & Thom.) were developed in vitro from shoot tip explants cultured on Murashige and Skoog (MS) medium containing only BAP or BAP with NAA, 3% sucrose and 0.8% agar. The addition of the following combination of growth substances 0.1mgLBAP and 0.05mgLNAA, caused an extensive proliferation of mul...

salvia leriifolia (lamiaceae) is endemic of khorasan and semnan province and an endangered plant. poor seed germination of this plant has a serious problem with the high production. the first step to improve this precious plant is to produce sterile plantlets in order to prepare explants of appropriate vigor. in this study,in vitro culture of salvia leriifolia zygotic embryo was performed throu...

Triploid plants of neem were obtained by immature endosperm culture. Immature seeds, at the early dicotyledonous stage of embryo development, is the best explant to raise endosperm callus on MS + NAA (5 mumol/L) + BAP (2 mumol/L) + CH (500 mg L-1). Maximum shoot bud differentiation from the endosperm callus occurred on MS + 5 mumol/L BAP. Shoots were multiplied by forced axillary branching and ...

Abstract Callus formation and somatic embryogenesis in potato varieties are effective ways to obtain an entire plant from a single cell. The implementation of is widely used improving materials creating new biotechnological varieties. In our research, the optimum level 1-naphthaleneacetic acid (NAA) 6-benzyl aminopurine (BAP) was determined callus tissue local Sarnav variety, Desiree variety ta...

Ginseng Jawa (Talinum paniculatum (Jacq.) Gaertn.) merupakan terna yang daunnya memiliki kandungan metabolit sekunder dan dapat digunakan sebagai obat. Beberapa penelitian kultur jaringan terkait sterilisasi penambahan zat pengatur tumbuh pada ginseng masih belum banyak dilakukan. Tujuan ini adalah mengetahui metode tepat serta melakukan optimalisasi Naphthalene Acetic Acid (NAA) Benzylaminopur...

abstract in vitro leaf explants of gf677 hybrid rootstock and rabi cultivar were placed on mql medium supplemented with bap at 0, 1, 2, 3 and 4 mg/l and naa at 0, 0.15 and 0.3 mg/l concentrations. the results revealed that bap, naa, genotype and their interactions had significant effect on both percentage and mean number of the regenerated shoots. for gf677, the highest percentage of shoot rege...