نتایج جستجو برای: cytochalasin blocked micronucleus assay

تعداد نتایج: 275230  

Journal: :Genetics and molecular research : GMR 2008
T D A Costa S Vieira S F Andrade E L Maistro

Cattley guava (Psidium cattleyanum Sabine) is a native fruit of Brazil that is popular both as a sweet food and for its reputed therapeutic properties. We examined whether it could damage DNA using the alkaline single-cell gel electrophoresis (comet assay) and the micronucleus test in leukocytes and in bone marrow cells of mice. P. cattleyanum leaf extract was tested at concentrations of 1000, ...

Journal: :The Journal of Cell Biology 1993
T A Gottlieb I E Ivanov M Adesnik D D Sabatini

Treatment with cytochalasin D, a drug that acts by inducing the depolymerization of the actin cytoskeleton, selectively blocked endocytosis of membrane bound and fluid phase markers from the apical surface of polarized MDCK cells without affecting the uptake from the basolateral surface. Thus, in MDCK cell transformants that express the VSV G protein, cytochalasin blocked the internalization of...

Journal: :Biomedical and environmental sciences : BES 2016
Ying Wu Shao Bin Gu Hao Li Jia Yi He Li Li Jian Bo Yang

We evaluated the antimutagenic effects of 10 kinds of bioactive phytochemicals and some phytochemical combinations against methotrexate (MTX)-induced genotoxicity by the umu test in Salmonella typhimurium TA1535/pSK1002 combined with a micronucleus assay. We observed that allicin, proanthocyanidins, polyphenols, eleutherosides, and isoflavones had higher antimutagenic activities than the other ...

Journal: :The Journal of pharmacology and experimental therapeutics 2000
T Udagawa J Yuan D Panigrahy Y H Chang J Shah R J D'Amato

Several previously identified inhibitors of angiogenesis have been epoxide-containing fungus-derived metabolites. We therefore hypothesized that novel epoxide-containing low molecular weight compounds structurally resembling known antiangiogenic agents may also exhibit antiangiogenic activity. Cytochalasin E was found to be a potent and selective inhibitor of bovine capillary endothelial (BCE) ...

Journal: :Mutagenesis 1996
M Matsui K Matsui Y Kawasaki Y Oda T Noguchi Y Kitagawa M Sawada M Hayashi T Nohmi K Yoshihira M Ishidate T Sofuni

Stevioside, a constituent of Stevia rebaudiana, is commonly used as a non-caloric sugar substitute in Japan. The genetic toxicities of stevioside and its aglycone, steviol, were examined with seven mutagenicity tests using bacteria (reverse mutation assay, forward mutation assay, umu test and rec assay), cultured mammalian cells (chromosomal aberration test and gene mutation assay) and mice (mi...

2013
Eduardo Padilla-Camberos Moisés Martínez-Velázquez José Miguel Flores-Fernández Socorro Villanueva-Rodríguez

The use of vegetal extracts requires toxicological and genotoxic evaluations to establish and verify safety before being added to human cosmetic, pharmaceutical medicine, or alimentary products. Persea americana seeds have been used in traditional medicine as treatment for several diseases. In this work, the ethanolic seed extract of Persea americana was evaluated with respect to its genotoxic ...

Journal: :Mutagenesis 2009
Michael Fenech Nina Holland Siegfried Knasmueller Sema Burgaz Stefano Bonassi

This report describes the aims, discussion and outcomes of the first Human Micronucleus collaborative project workshop on the buccal micronucleus assay. It was agreed at the workshop that three activities should be initiated as soon as possible namely (i) a method for collection of databases, (ii) writing of a protocol based on the most commonly used and best validated procedures and (iii) an i...

2017
Sanjay Singh A. S. Yadav

The Buccal Micronucleus Cytome (BMCyt) in exfoliated buccal epithelial cells is a minimally invasive method for assessing chromosomal instability, DNA damage and cell death. It has been widely used in occupational and lifestyle related studies. For the present study 63 male subjects were taken out of which 32 were vegetarian and 31 subjects were nonvegetarian matched with respect to their age, ...

Journal: :Journal of cell science 1993
A Sonnenberg A A de Melker A M Martinez de Velasco H Janssen J Calafat C M Niessen

Keratinocytes attach to an underlying basement membrane by adhesion junctions called hemidesmosomes. We have characterized a cell line, RAC-11P/SD, established from a murine mammary tumor, which differentiates into squamous epithelium and forms well defined hemidesmosomes. These hemidesmosomes contain the integrin alpha 6 beta 4 as well as the hemidesmosomal plaque proteins BP230 and HD1 and ar...

Journal: :Arhiv za higijenu rada i toksikologiju 2014
Elizabeta Galić Antonija Tadin Nada Galić Vilena Kašuba Marin Mladinić Ružica Rozgaj Dolores Biočina-Lukenda Ivan Galić Davor Zelježić

The purpose of this study was to evaluate the genotoxic potential of components leached from two conventional self-curing glass-ionomer cements (Fuji IX and Ketac Molar), and light-curing, resin modified glass-ionomer cements (Vitrebond, Fuji II LC). Evaluation was performed on human lymphocytes using alkaline and hOGG1 modified comet, and micronucleus assays. Each material, polymerised and unp...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید