Compared to cell-based protein expression, cell-free protein synthesis (CFPS) offers several advantages including a greater control over system additives. This control is further enhanced with a CFPS system called the Protein synthesis Using Recombinant Elements (PURE) system, which consists of 108 purified transcriptional and translational elements. With the PURE system, all elements are known...

Balaenid whales perform long breath-hold foraging dives despite a high drag from their ram filtration of zooplankton. To maximize the volume of prey acquired in a dive with limited oxygen supplies, balaenids must either filter feed only occasionally when prey density is particularly high, or they must swim at slow speeds while filtering to reduce drag and oxygen consumption. Using digital tags ...

OBJECTIVE Leptin, a hormone present in breast milk, is involved in energy regulation and metabolism. The purpose of this investigation was to determine whether leptin is present in either preterm breast milk (PBM) or preterm formula (PF). The effects of delivery methods and pasteurization on leptin levels also were evaluated. METHODS PBM samples were obtained from 29 mothers who delivered inf...

The activity of sucrose-sucrose-fructosyltransferase (SST), a vacuolar enzyme strongly induced by light in excised leaves of barley (Hordeum vulgare L.), rapidly declined even in continuous light upon feeding of cycloheximide (CHI). The rate of decline was similar to that observed in light-treated leaves that were placed into darkness, in the presence or absence of CHI. The protease inhibitor l...

Abstract Extrauterine growth restriction is a common complication of preterm birth and associated with reduced lean long-term morbidities. We previously showed that intermittent pulses leucine during continuous orogastric feeding increases mTORC1 signaling to protein synthesis in skeletal muscle neonatal pigs born at term. The objective this study was determine the extent which would promote by...

In article number 2300031, Hua-Bing Li, Zhou-Hua Jiang, and co-workers depict a schematic diagram illustrating the continuous casting process of slabs, along with corresponding temperature distribution after steel strip feeding. This analysis examines progressive changes in morphology, focusing on successive stages rapid cross-sectional area expansion, subsequent sharp decline, oscillation, ult...