Abstract Background The abundance of human milk leukocytes, including neutrophils, changes in response to the health status mothers and their infants. Elevated leukocyte counts occur during infant infection return baseline levels upon recovery. However, it is unclear how can affect neutrophil phenotypes concentration fatty acids mothers’ milk. Objectives This study investigates association betw...

OBJECTIVE To assess the functional status of the IL-15/IL-15Ralpha cytokine system in different leucocyte subsets from patients with systemic lupus erythematosus (SLE). METHODS Eighteen patients with SLE (10 with inactive and eight with active disease) and 14 healthy individuals were studied. Serum levels and in vitro production of IL-15 were determined. In addition, the expression of IL-15 r...

OBJECTIVE To establish an easy and practical assay for identifying systemic interferon (IFN) type I bioactivity in patients with primary Sjögren's syndrome (pSS). The IFN type I signature is present in over half of the pSS patients and identifies a subgroup with a higher disease activity. This signature is currently assessed via laborious expression profiles of multiple IFN type I-inducible gen...

Glycosaminoglycans (GAG) are a group of negatively charged molecules that have been shown to bind and directly regulate the bioactivity of growth factors and cytokines such as basic fibroblast growth factor, transforming growth factor-beta, IL-7, and interferon-gamma. The ability of GAG to interact with human IL-10 (hIL-10) and the effect of these interactions on its biologic activity were anal...

Cellular sources of IL-23 and IL-17A driving skin inflammation in psoriasis remain unclear. Using high-dimensional unsupervised flow cytometry analysis, mononuclear phagocytes T cells were examined the same lesions patients before during guselkumab (IL-23p19 blocker) or secukinumab (IL-17A treatment. Among CD11c+HLA-DR+ phagocytes, CD64brightCD163−CD14brightCD1c−CD1a‒ inflammatory monocyte‒like...