BACKGROUND Bovine viral diarrhea (BVD) is an economically important disease of cattle distributed worldwide. Diagnosis of BVD relies on laboratory-based detection of its viral causing agent or virus specific antibodies and the most common laboratory method for this purpose is Enzyme-Linked Immunosorbent Assay (ELISA). OBJECTIVES The current study was aimed to develop a simple indirect ELISA t...

Hantaan virus is the causative agent of severe hemorrhagic fever with renal syndrome. Clinical surveillance for Hantaan virus infection is unreliable, and laboratory verification is essential. The detection of virus-specific immunoglobulin M (IgM) and IgG in serum is most commonly used for the diagnosis of hantavirus infection. Testing of oral fluid samples instead of serum offers many advantag...

Hepatitis B virus (HBV) DNA diagnosis plays a critical role in the transmission of HBV infections. Accurate protocols detection will reduce spread from blood donors. Therefore, this research compared detecting potentials three different detection. Blood 200 donors at University college bank Ibadan were sampled and tested for using ABON™ HBsAg Surface Antigen Tests (strip test), Monolisa TM ULTR...

Hepatitis B virus (HBV) infection is of global public health concern. Among various serological tests used for the diagnosis and screening of HBV infection, the enzyme-linked immunosorbent assay (ELISA) to detect hepatitis B surface antigen (HbsAg) is most widely used. The present study was designed to develop and standardize a cost effective in-house ELISA for the detection of HbsAg and compar...

Two hundred and fourteen human faecal specimens were examined for detecting the presence of rotavirus by direct electron microscopy, antibody capture electron microscopy and ELISA. Twenty nine samples were positive with one of the three methods. Thirtyeight percent were positive with all three methods, 72.4% with ELISA, 62% with antibody capture electron microscopy and 55% with direct electron ...

For evaluating the antigen content in recombinant Hepatitis B Vaccine (Monovalent and Pentavalent vaccines), National Control Laboratory, Kasauli has taken an initiative to develop a uniform method for evaluation of antigen content of Hepatitis B vaccine. All the manufacturers in India are having different method for evaluating Relative Potency of Hepatitis B Vaccine. In the present study the s...

BACKGROUND Without appropriate culture systems for hepatitis E virus (HEV), sufficient natural viral proteins are difficult to generate for use in serological tests. Therefore, it is important to produce large amounts of HEV recombinant proteins in an economical way. The present study developed ELISAs using 2 truncated forms of the HEV open reading frame (ORF) 2 protein in order to detect anti-...

was quantified by integration using EZLogicTM integration software (Bio-Rad). The clearance of host cell proteins (HCP) and double-stranded DNAs (dsDNA) was determined by a CHO-CM HCP ELISA kit (Cygnus Technologies) and Quant-iT dsDNA high-sensitivity assay kit (Life Technologies Corporation), respectively. mAb1 concentration was determined by UV absorption at 280 nm using a coefficient of 1.4 ...

9:00 Welcome Annemiek Geluk LUMC ‘Development of novel T cell assays for detection of M. leprae infection’ Linda Oskam, KIT, The Netherlands ‘New and improved opportunities for serological KIT, testing in leprosy’ John Spencer, CSU, USA ‘Use of Western blot, ELISA and protein microarrays to define disease-state-specific antigenic profiles in leprosy patients’ Sang-Nae Cho, YUMC, Korea ‘Enzyme i...

An ELISA was developed using chicken cystatin as a capture agent for the immunodiagnosis of paragonimiasis and fascioliasis. The assay detected specific antibodies to fluke cysteine proteinases without the need for purified proteinases. An ELISA plate was sensitized with chicken egg white cystatin, incubated with excretory-secretory (ES) products of adult flukes, and standard ELISA procedures w...