In this paper, we studied polyetheneglycol (PEG) pretreatment effect on the mature embryo culture in vitro by using barley (Hordeum vulgare L.) seeds. Meanwhile, we analyzed and assayed its mineral element and endogenous hormone level. The experimental results were as follows: (1) PEG-6000 imbibition could obviously slow down the water timecourse absorbed by barley seeds; (2) 10% PEG-6000 treat...

Two strains of callus have been isolated from bean hypocotyl and grown on a defined maintenance medium supplemented with 2 mg/l. 2:4-dichlorophenoxyacetic acid (2:4D) and 2% sucrose. Root initiation was observed in one strain and formation of nodules containing xylem and phloem in both strains after transfer to an induction medium supplemented with 1 mg/l. naphthyleneacetic acid, 0-2 mg/l. kine...

Anti-inflammatory activities of methanolic extract of callus and intact plant part of Stevia rebaudiana(Bert.) Bertoni was studied for the first time. Acute inflammation model, carrageenan induced rat hind paw edema model was employed to investigate the anti-inflammatory activity. The methanolic extract from fresh callus cultures of Stevia rebaudianacultured on Murashige and Skoog (MS) medium f...

Scented rose species are very important in the genus Rosa due to their use in highly-prized essential oil production and as garden roses. The fragrance of the rose flower (attar) captured by extraction is one of the most valuable flavor and fragrance products. A comprehensive study of the papers published in the field of in vitro culture and biotechnology of these species, particularly Damask r...

Datura metal L is an important medicinal plant containing the alkaloids like scopalamine(hyoscine),hyoscyamine(atropine) scutellaerin etc .The total alkaloids present in the callus were higher than the amount of alkaloids present in leaves .For the higher production of alkaloids the medium plays avital role.The addition of L-arginine were enhanced the alkaloid production The higher production o...