The purpose of this study was to assess the effects of the addition of calcium to University of Wisconsin solution in long-term myocardial perfusion. In a heterotopic heart transplantation model, performed in pigs, the donor heart was preserved for 24 hours by means of continuous perfusion in this solution, without (24hUW group) or with calcium, 2.4 mmol/L (24hUW+Ca). During this period, the ox...

BACKGROUND Brief myocardial ischaemia has been demonstrated to result in mechanical and coronary endothelial dysfunction, in which calcium may play a role. We examined whether the mechanical and vascular responses to calcium are altered in postischaemic, reperfused myocardium. METHODS Regional myocardial oxygen consumption (MVO2), mechanical function and coronary blood flow (CBF) in response ...

Abstract Soil P testing has been widely used to predict crop yields, uptake, and fertilizer demands in agriculture. Diffusive gradients thin films (DGT) provides a zero-sink soil test which mimics diffusion-controlled plant uptake previously found availability crops better than conventional quantity-based tests highly weathered Australian, though not European soils. Here we tested the performan...

The effect of sodium lactate is compared with sucrose + sorbitol + sodium tri-poly phosphate as cryoprotectant on gel forming ability & protein denaturation of croaker surimi during frozen storage at -20±2°C for 90 days was evaluated. The quality of Croaker surimi with 6% (w/v) sodium lactate was examined in terms of biochemical parameters of muscle protein, thaw drip, gel strength and calcium ...

Midland painted turtles from Michigan were submerged at 3 degrees C in normoxic and anoxic water. In predive, and in turtles submerged for up to 150 days, plasma PO2, PCO2, pH, [Cl-], [Na+], [K+], total Mg, total Ca, lactate, glucose, and osmolality were measured; hematocrit and mass were determined, and plasma [HCO3-] was calculated. Anoxic turtles developed a severe metabolic acidosis, accumu...

water in the citric acid cycle. Peritoneal dialysate contains a racemic mixture of both D and L isomers of lactate and mammals lack D-lactic dehydrogenase and are slower in metabolising D-lactate.' There is only one previously reported case of lactic acidosis in a patient receiving peritoneal dialysis, which like our case was related to metformin.4 Severe lactic acidosis has a mortality of abou...

BACKGROUND Streptococcus pneumoniae causes serious diseases such as pneumonia and meningitis. Its major pathogenic factor is the cholesterol-dependent cytolysin pneumolysin, which produces lytic pores at high concentrations. At low concentrations, it has other effects, including induction of apoptosis. Many cellular effects of pneumolysin appear to be calcium dependent. METHODS Live imaging o...