Objective(s): Adeno-associated virus type 2 (AAV2) vectors are widely used for both experimental and clinical gene therapy. A recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. In this study, a fast and simple method is presented to generate AAV2 vector helper plasmids encod...

The objectives of the present research were to evaluate the variability induced by gamma-irradiation among a population of M8 sunflower mutant lines and to identify molecular markers associated with different seed germination traits. Experiments were carried out under well watered and water-stressed conditions using a randomized blocks design, with three replications. The studied traits consist...

Background and Aims: Q59L mutant of L-asparaginase enzyme from Escherichia coli (E. coli) has been introduced with lower side effects. This version of the enzyme might have potential applications in the treatment of leukemia patients. We utilized SHuffle T7 strain of E. coli, to produce the mutant enzyme in the presence of chaperone molecules. Materials and Methods: Q59LAsp gene was cloned in...

In a murine model of acute fatal pneumonia, we previously showed that nasal immunization with a live-attenuated aroA deletant of Pseudomonas aeruginosa strain PAO1 elicited LPS serogroup-specific protection, indicating that opsonic Ab to the LPS O Ag was the most important immune effector. Because P. aeruginosa strain PA14 possesses additional virulence factors, we hypothesized that a live-atte...

using mutation specific pcr assay and analysis of position 108 amino acid in dihydrofolate reductase-thymidilate synthase gene in 70 plasmodium falciparum isolates of malarial patients from southern areas of iran, showed 4 different situations: iwild type (108-ser) ii-mutant type (108-thr) iii-mutant type (108-asn) and iv-mixed wild and mutant type (108-ser+thr) with frequency of 58(82.8%), 2(2...

Low threshold Ca2+ currents mediated by T-type channels underlie burst spike activities of relay neurons in the thalamus. We have previously reported that knock-out mice for T-type channels show an enhanced nociceptive response to visceral pain, accompanied by an increase in tonic spikes in the absence of burst spikes in thalamic relay neurons. These results raised a possibility that T-type cha...

objective(s): adeno-associated virus type 2 (aav2) vectors are widely used for both experimental and clinical gene therapy. a recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. in this study, a fast and simple method is presented to generate aav2 vector helper plasmids encodin...

conclusions expression levels of 1,426 genes were significantly up-regulated in the mutant compared to wt. furthermore, 301 genes were down-regulated with false discovery rates (fdr) of < 0.001 and absolute value of log2 ratio of ≥ 1. results a total of 4,182,944 and 4,182,021 clean tags were obtained from the wt and a nonpathogenic mutant stain (bct89), respectively, and 10,410 differentially ...

conclusions this study showed that the presence of mutation at codons rtl80v, rtl180m and rtm204v in a, b and c domains is associated with higher viral load and resistance to lamivudine (3tc) respectively. results about 6% (3 of 50) of samples were hbeag positive and 94% (47 of 50) of patients were hbeag negative by elisa method. the patients' alt level was between 16 and 95 iu/l with the mean ...