The antiserum against mouse growth hormone (MGH) was produced by immunizing monkeys and some basic immunological properties of the antiserum were studied. The antiserum formed single precipitin lines in agar gel double diffusion plate against both MGH preparation and the crude extract of mouse anterior pituitaries, but reacted with neither normal mouse serum nor the crude extracts of several vi...

Mastitis is known to be an economically important disease hampering desired progress in the dairy industry. Mastitis caused by Streptococci is of the subclinical type.Early detection of mastitis is of paramount importance to avoid permanent damage to the udder and loss of milk.The presently detection methods of Electrical Conductivity test (EC) and Somatic Cell Count (SCC) for subclinical bovin...

BACKGROUND The Cupressaceae are an important cause of pollinosis, particularly in Mediterranean countries. Cypress pollen allergenic extracts are difficult to produce since they have a low protein and a high carbohydrate content and consequently accurate standardization of these extracts is essential for diagnosis and immunotherapy. METHOD Natural Cup s 1 was purified by a combination of hydr...

OBJECTIVES Brucellosis is one of the most common zoonoses in the world, and occurs mainly in farmers, slaughterhouse workers, and veterinarians via direct or indirect contact with infected animals or their products. The clinical symptoms of human brucellosis are nonspecific, such as fever, headache, chills, and sweating. Diagnosis and treatment of brucellosis requires laboratory tests. Although...

Background: Newcastle disease virus (NDV) is a dangerous viral disease, infecting a broad range of birds, and has a fatal effect on the poultry industries. The attachment and consequently fusion of the virus to the host cell membrane is directed by the two superficial glycoproteins, the hemagglutinin-neuraminidase (HN) and the fusion (F) which is considered as the important tar...

two hundred and thirty one leaf samples of virus infected and mosaic and as well dwarf mosaic symptoms showing maize plants from various corn fields in tehran province were collected during the growing season 2003. by performing serological tests of das-elisa, diba and tpia, using scmv, mdmv, srmv and jgmv antisera, only scmv was detected in samples, with all samples reacting strongly to the sc...

As a fundamental experiment for the study on the role of growth hormone in mouse mammary gland, some immunological properties of rabbit antiserum to mouse growth hormone (MGH) were studied. Antiserum was obtained by immunizing New Zealand White male rabbits with MGH preparation extracted from mouse anterior pituitaries. The precipitin titer of the antiserum by precipitin ring test against MGH p...

Classical swine fever virus (CSFV) C-strain "Riems" escape variants generated under selective antibody pressure with monoclonal antibodies and a peptide-specific antiserum in cell culture were investigated. Candidates with up to three amino acid exchanges in the immunodominant and highly conserved linear TAV-epitope of the E2-glycoprotein, and additional mutations in the envelope proteins ERNS ...

A biotinylated 3ABC recombinant protein was developed and used in a competitive ELISA (cELISA) to detect foot-and-mouth disease virus (FMDV) antibodies in cattle, sheep and pigs. In this report, we describe the cloning and expression of 3ABC protein in Escherichia coli cells as fusion protein with 6xHis and biotin. This cELISA uses streptavidin to capture bacterially expressed and in vivo bioti...

A human high mobility group box 4 (hHMGB4) expression construct (pET‑28a/hHMGB4) was generated by cloning the hHMGB4 full‑length cDNA in the expression vector pET‑28a(+). The hHMGB4 fusion protein with His6‑Tag was prepared using E.coli BL21 (DE3) transformed with pET‑28a/hHMGB4 and purified via preparative SDS‑PAGE plus electroelution. Immunization of rabbits with the purified hHMGB4 generated...