Between August 8, 1974, and November 1, 1978, calls reporting 100 cases of mushroom poisoning were received by the National Institutes of Health. Thioctic acid was sent (under IND # 9957) for the treatment of seventy-five of these patients thought to have ingested mushrooms containing alpha-amanitin. Sixty-seven patients recovered, and eight died. The lapse of time between ingestion of mushroom...

RNA metabolism and content in AKR-2B cells has been studied extensively in the G0/Gi-arrested state and following the stimulation to proliferate. The purpose of the present study was to examine selected parameters of RNA metabolism in a chemically transformed derivative of the AKR-2B cell, AKRMCA, at a G, arrest point and following the stimulation to undergo DMA synthesis. Flow microfluorometri...

T structure of 10-subunit 0.5-MDa yeast RNA polymerase II (pol II), recently determined at 2.8 Å resolution, reveals the architecture and key functional elements of the enzyme (1). The two largest subunits, Rpb1 and Rpb2, lie at the center, on either side of a nucleic acid-binding cleft, with the many smaller subunits arrayed around the outside. Rpb1 and Rpb2 interact extensively in the region ...

We have developed soluble extracts from Neurospora crassa capable of accurately initiating the transcription of cloned Neurospora protein-encoding genes by RNA polymerase II in vitro. The genes encoding glutamate dehydrogenase (am) and histones H3 and H4 were transcribed by the extracts, and transcription was sensitive to alpha-amanitin at 1 mg/ml. The 5' heterogeneity of the in vitro initiatio...

Although rare, death from amanitin exposure poses a significant health risk and a diagnostic challenge to the clinician due to its rarity. This is one of the few conditions to be voluntarily reported by healthcare professionals. No antidote exists for this poisoning and, perhaps due to its rarity or lack of attention, the United States has lagged behind Europe for almost three decades in treatm...

incorporation of [%]methionine into protein has recovered to control levels. We therefore shifted the exposure to anisomycin putting it either before 5-HT application (by 12 h) or after the last 5-HT application (0.5 h or 4 h). In each case, anisomycin had no statistically significant effect on the 5-HT-induced long-term facilitation (65 k lo%, n = 4; 51 f 19%,n = 4;90 k 32%,n = 4,respectively)...