As illustrated in the preceding paper (Firestone, G. L., and Heath, E. C. (1981) J. Biol. Chem. 256, 13961403), the de novo synthesis of a cell surface-localized glycoprotein that exhibits alkaline phosphatase activity was induced by treatment of mouse L-cell cultures with dibutyryl cyclic AMP. When the exposure of L-cell cultures to dibutyryl cyclic AMP was carried out in the presence of inhib...

BACKGROUND Alkaline amylase has significant potential for applications in the textile, paper and detergent industries, however, low yield of which cannot meet the requirement of industrial application. In this work, a novel ARTP mutagenesis-screening method and fermentation optimization strategies were used to significantly improve the expression level of recombinant alkaline amylase in B. subt...

Botulinus toxin resists a degree of acidity equivalent to that of the stomach even when exposed thereto for 24 hours at 37 degrees C. It is less resistant to alkali, however, as shown by the fact that in a medium of weakly alkaline reaction its potency is reduced to less than one-tenth in 24 hours. It is unaffected by peptic and tryptic digestion. Because of its instability in mildly alkaline s...

Alkaline treatment of the Ectothiorhodospira sp. light harvesting system II induces monomerisation of the bacteriochlorophylls and a bleaching of the carotenoid absorption bands in the visible region. Concomitantly, the maximum of absorption observed around 373 nm shifts towards 354 nm. This shift does not result from the Soret band but from a change of the absorption properties of the caroteno...

The degradation behavior of cefquinome sulphate in alkaline medium at different temperatures was investigated using both first derivative spectrophotometric and HPLC methods. The drug degradation was found to be pH and temperature dependant. The pH-rate profile indicated a first order dependence of Kobs on [OH(-)] at pHs ranging between 9 and 11. Arrhenius plot obtained at pH 10 was linear betw...

Hydrogen peroxide has been identified as a stable intermediate of the electrochemical oxygen reduction reaction on various electrodes including metal, metal oxide and carbon materials. In this article we study the hydrogen peroxide oxidation and reduction reactions in alkaline medium using a rotating disc electrode (RDE) method on oxides of the perovskite family (LaCoO3, LaMnO3 and La0.8Sr0.2Mn...