background: agriculture is an important sector for the malaysian economy. the use of pesticides in agriculture is crucial due to its function in keeping the crops from harmful insects. children living near agricultural fields are at risk of pesticide poisoning. objective: to evaluate the genotoxic risk among children who exposed to pesticides and measure dna damage due to pesticides exposure. m...

The comet assay has been widely used to measure a range of cellular responses to DNA damage and has found applications in genotoxicity studies, bio-monitoring, ecological testing and in the study of human disease. This review discusses how the comet assay has been applied to the study of DNA damage and repair associated with cancer. The potential of the assay as a tool for predicting an individ...

The vertical comet assay is a relatively new technique for quantitatively measuring radiation induced DNA damage in cells. The vertical comet assay provides potential for measuring DNA strand breaks in a dense monolayer of plated cells. This paper explores the successes and challenges of developing a vertical comet assay system for use with the current Microbeam II alpha particle source at RARA...

To monitor genotoxicity in small bodies of water (e.g., creeks, ponds, and drainage ditches) we examined tadpole erythrocytes of two species: Rana clamitans and Rana pipiens,using the alkaline single cell gel DNA electrophoresis (SCG) or "comet" assay. This approach involves detection, under alkaline conditions, of cell DNA fragments which on electrophoresis migrate from the nuclear core, resul...

butachlor (n-(butoxymethyl)-2-chloro-n-2, 6-diethylacetanillide) is widely used chloroacetanilide herbicide in asian region, especially in the north of iran. several studies have been reported on toxicity of butachlor, but little information is accessible on dna damaging potential. thus, the present study was undertaken to investigate of dna damage by butachlor in cyprinuscarpio(pisces: cyprini...

malathion (s-(1, 2-dicarboethoxyethyl) o, o-dimethyl phosphorodithioate) is one of the organophosphate pesticides comprehensively used in agriculture fields throughout the world. in spite of widely used, little information are available about dna damage in aquatic organisms. therefore, the present study carried out to investigation of dna damage induced by malathion in cyprinus carpio (pisces: ...

objective: achillea is a traditional medicinal herb which contains different phenol and flavonoid compounds that are responsible for achillea pharmacological effects. we aimed to determine phenol and flavonoid contents, besides antioxidant activities of different extracts from achillea eriophoraa (a. eriophora) dc. and achillea biebersteinii (a. biebersteinii) afan. (endemic species in iran) an...

background: cadmium is one of the most toxic heavy metals in our environment having a very strong ability to accumulate in body organs, especially in kidney. the present study was done to determine the genotoxicity and cytotoxicity in kidneys of rats exposed to cadmium. methods: male rats (n=30), kept in standard conditions were used in this study. the animals were randomly divided into 2 group...

introduction: silymarin is obtained from silybum marianum (milk thistle), an edible plant that has long been used medicinally for the treatment of liver-related disorders. silymarin is a powerful hepatoprotective and antioxidant but the anticlastogenic activity, which is an important aspect of its cancer chemoprevention is not known hence the present investigation was carried out to study its a...

The electrophoresis pattern of apoptotic cells detected by the comet assay has a characteristic small head and spread tail. This image has been referred to as an apoptotic comet, but it has not been previously proven to be apoptotic cells by any direct method. In order to identify this image obtained by the comet assay as corresponding to an apoptotic cell, the frequency of appearance of apopto...