background fish cell lines are advantageous alternatives to mammalian cell lines for carrying out in vitro research. they are used extensively for the study of fish biology, physiology and toxicology, and more recently for germplasm conservation of important fish species. objectives the present study aims to establish and characterize a novel fish cell line from muscle tissue of wallago attu ca...

Beclin 1 is a promoter gene for autophagy as well as a key factor for regulating tumor cell growth and death. Allelic deletion of Beclin 1 has been observed in certain triple-negative breat cancer (TNBC) cells, and it might be associated with increased proliferation and invasion in TNBC cells. In this study we investigated the relationship between Beclin 1 expression and prognosis for TNBC pati...

Most normal cells have two centrosomes that form bipolar spindles during mitosis, while cancer cells often contain more than two, or "supernumerary" centrosomes. Such cancer cells achieve bipolar division by clustering their centrosomes into two functional poles, and inhibiting this process then leads to cancer-specific cell death. A major problem with clinically used anti-mitotic drugs, such a...

In this work, the compound 2 named ethyl (1,1-dichloro-1a,5,5,7-tetramethyl-8-oxo-1a,2,3,4,5,5a,8,9-octahydro-1H-benzo[a]cyclopropa[b][7]annulen-6-yl)glycinate (C20H29Cl2NO3) was synthesized from ?,?-unsaturated sesquiterpene ketone 1 using azido-Schmidt reaction catalyzed by titanium tetrachloride (TiCl4). The structure of newly enaminone fully identified its HRMS, FT-IR, UV-Visible, NMR (1H &...

the objective of the study was to evaluate the cytotoxic effects of aqueous extract of cucumis melo fruit on human prostate cancer cell line (pc-3) using mtt and neutral red assays. the crude aqueous extract of c. melo was prepared by cold maceration method, filtered, concentrated and tested on pc-3 cell line. dose-dependent cytotoxic activities were exhibited on human prostate carcinoma pc-3 c...

The aim of this study was to evaluate and compare the cytotoxicity induced by two resin-based sealers, 2Seal and AH Plus, in two osteoblast-like cell lines, MG-63 and Saos-2. Using sterile discs of both sealers in complete media, 24- and 72-h extracts were prepared. The extracts were exchanged with Saos-2 or MG-63 cell culture media at 75% confluence, and after 24 h incubation, cell viability t...

On the course of screening program for new bioactive compounds, the present study was carried out on the crude extract of Penicillium janthinellum 62. The crude extract of P. janthinellum 62 was fractionated on silica gel column and Sephadex-LH20, ten active fractions were produced. Fraction 7 showed highest antitumor activity, which then purified using preparative TLC and produced six sub-frac...

Target-specific treatment modalities are currently not available for triple-negative breast cancer (TNBC), and acquired chemotherapy resistance is a primary obstacle for the treatment of these tumors. Here we employed derivatives of BT-549 and MDA-MB-468 TNBC cell lines that were adapted to grow in the presence of either 5-Fluorouracil, Doxorubicin or Docetaxel in an aim to identify molecular p...