نتایج جستجو برای: 2dg electrophoresis

تعداد نتایج: 50889  

Journal: :The Journal of Experimental Medicine 1980
C G Ragsdale W P Arend

Human monocytes cultured on surface-bound immune complexes exhibited a loss of ability to form rosettes with IgG-sensitized sheep erythrocytes (EA). This loss was not a result of inhibition of Fc receptors by solubilized complexes nor of release of soluble factors by the cells. Loss of EA rosetting was not prevented by culture of monocytes at 4 degrees C, or by treatment with colchicine, cytoch...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 1988
S Caldecott-Hazard J Mazziotta M Phelps

14C-2-Deoxyglucose (2DG) was used to investigate changes in the rate of cerebral metabolism in 3 rat models of depressed behavior. The models had already been established in the literature and were induced by injections of alpha-methyl-para-tyrosine, withdrawal from chronic amphetamine, or stress. We verified that exploratory behaviors were depressed in each model and that an antidepressant dru...

Journal: :JCI insight 2017
Annelise Y Mah Armin Rashidi Molly P Keppel Nermina Saucier Emily K Moore Joshua B Alinger Sandeep K Tripathy Sandeep K Agarwal Emily K Jeng Hing C Wong Jeffrey S Miller Todd A Fehniger Emily M Mace Anthony R French Megan A Cooper

NK cell activation has been shown to be metabolically regulated in vitro; however, the role of metabolism during in vivo NK cell responses to infection is unknown. We examined the role of glycolysis in NK cell function during murine cytomegalovirus (MCMV) infection and the ability of IL-15 to prime NK cells during CMV infection. The glucose metabolism inhibitor 2-deoxy-ᴅ-glucose (2DG) impaired ...

Journal: :American journal of physiology. Regulatory, integrative and comparative physiology 2009
C Kay Song Gary J Schwartz Timothy J Bartness

The origins of the sympathetic nervous system (SNS) innervation of white adipose tissue (WAT) have been defined using the transneuronal viral retrograde tract tracer, pseudorabies virus. Activation of this SNS innervation is acknowledged as the principal initiator of WAT lipolysis. The central control of WAT lipolysis may require neural feedback to a brain-SNS-WAT circuit via WAT afferents. Ind...

Journal: :Biomedical Journal of Scientific and Technical Research 2023

The most common technique for sorting, identifying, and purifying nucleic acid fragments is agarose gel electrophoresis. performance of the electrophoresis could be impacted by buffers’ composition, ionic strength, pH characteristics [1].

Journal: :Nature 1964

2016
RAJ KUMAR MONGRE SIMRINDER SINGH SODHI NEELESH SHARMA MRINMOY GHOSH JEONG HYUN KIM NAMEUN KIM YANG HO PARK YOUNG GYU SHIN SUNG JIN KIM ZHANG JIAO JIAO DO LUONG HUYNH DONG KEE JEONG

Tumor initiating cancer stem-like cells (TICSCs) have recently become the object of intensive study. Human-Lipocalin-2 (hLCN2) acts as a biomarker for cancers. The aim of the present study was to explore new insights regarding the potential role of LCN2 in inducing epithelial to mesenchymal transition (EMT) by transfecting LCN2 into CD133+-A549-TICSCs and its cross-talk with the NF-κB signaling...

Journal: :Annals of the New York Academy of Sciences 2001
G S Roth D K Ingram M A Lane

Dietary caloric restriction (CR) is the only intervention conclusively and reproducibly shown to slow aging and maintain health and vitality in mammals. Although this paradigm has been known for over 60 years, its precise biological mechanisms and applicability to humans remain unknown. We began addressing the latter question in 1987 with the first controlled study of CR in primates (rhesus and...

Journal: :Analytica Chimica Acta 2021

• Capillary electrophoresis is well adapted for amino acids analysis. associated to fluorescence results in nanomolar concentration detections. conductimetry able be used most applications. mass spectrometry a very useful tool metabolomic Enantio-separations of are easily obtained using capillary electrophoresis.

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