147 Rev Esp Cardiol. 2005;58(10):1246-8 1247 ment by those who defend the two-stage procedures was not especially notable in our case. Circulatory arrest with deep hypothermia avoids subjecting the liver and kidneys to periods of warm ischemia. Other advantages of this technique are the physical benefits for the patient and economic benefits for the system, as the surgery is performed in a sing...

In this study, we report the development and validation of a real-time polymerase chain reaction (PCR) assay using a Taqman-labeled probe for the detection of Mycoplasma gallisepticum (MGLP assay). The MGLP assay was highly specific with a detection limit of 25 template copies per reaction and a quantification limit of 100 template copies per reaction. Validation of the assay was completed with...

OBJECTIVE To determine the coverage provided by the Kimberley Diabetic Retinopathy Screening Program and evaluate the impact of the Kimberley diabetic eye health coordinator (KDEHC) position using an evidence-based approach. DESIGN Retrospective audit. SETTING Primary care services in the Kimberley region of Western Australia. PARTICIPANTS Individuals with diabetes mellitus who underwent ...

OBJECTIVE Investigate differences in the infectious aetiology, health seeking behaviour, and provider practices with regard to diarrhoeal illness among children presenting to urban versus rural clinics in Western Kenya. DESIGN Laboratory-based, passive surveillance. SETTING The urban portion of the study was conducted at the paediatric outpatient clinic of Nyanza Provincial Hospital in Kisu...

Efficient solution methods are investigated in this paper for solving the linear system of equations resulting from the recently developed boundary element method ~BEM! for the coupled structural acoustic analysis @S. H. Chen and Y. J. Liu, J. Acoust. Soc. Am. 106, Pt. 1, 1247–1254 ~1999!#. An iterative solver, namely, the quasiminimal residual method ~QMR!, is selected among others and found t...

BACKGROUND Serological studies indicate that evidence of coeliac disease (CD) exists in about 1% of all children, but we lack estimates of current diagnostic patterns among children and how they vary by socioeconomic group. METHODS We identified all children aged 0-18 years between 1993 and 2012 who were registered with general practices across the UK that contribute to a large population-bas...

Ewing sarcoma is one of themost frequent bone cancers in adolescence. Althoughmultidisciplinary therapy has improved the survival rate for localized tumors, a critical step is the development of new drugs to improve the long-term outcome of recurrent and metastatic disease and to reduce side effects of conventional therapy. Here, we show that the small molecule reactivation of p53 and induction...

Apoptosis is a highly regulated cellular process that functions to remove undesired cells from multicellular organisms. This pathway is often disrupted in cancer, providing tumours with a mechanism to avoid cell death and promote growth and survival. The putative tumour suppressor, SASH1 (SAM and SH3 domain containing protein 1), has been previously implicated in the regulation of apoptosis; ho...

OBJECTIVES To assess the various recruitment strategies used by medical researchers and their response rates. METHODS The observational study, part of a larger retrospective cohort, was done at the Aga Khan University Hospital, Karachi, Pakistan, from May 2008-December 2010, covering a period from 1999 to 2005. We used a multi-mode contact approach for including participants in the study. Thi...