Procedure and Notes • Use only well mixed, non-centrifuged urine, which should not be older than 4 hours. First morning urine is recommended. Protect the samples from light. • If the samples cannot be tested immediately, they should be stored at 2...4°C and brought to room temperature (15...25°C) before testing. • Collect specimen in clean, well rinsed containers, free of detergents. Do not add...

Urine p-aminophenol (PAP) concentration serves as a biological marker for occupational exposures to aniline. We report the development of a rapid, simple spectrophotometric method for quantification of urine PAP concentration using a chemical autoanalyzer (Olympus Reply). The method involves oxidative coupling of PAP with an aromatic compound, xylenol, that contains an electron-donating group, ...

A 67-year-old woman presented to the emergency department in congestive heart failure. She also had a history of transverse myelitis, which had caused her to be bedbound with an indwelling urinary catheter. During the physical examination, the urine in her Foley tubing and bag were noted to be bright purple. When asked, she replied that the urine had turned purple several weeks earlier. She den...

We report on a patient with glucose-6-phosphate dehydrogenase (G6PD) deficiency who developed acute hemolytic anemia after having received an injection of Ginkgo biloba for dementia prophylaxis without medical advice. She suddenly developed general malaise, generalized yellowish skin color, and tea-colored urine. Intravenous fluid infusion and cessation of G. biloba quickly relieved her clinica...

We investigated the formation of delta-aminolevulinic acid (ALA)-pyrrole and subsequent color development used in determining ALA in urine. Of three condensation reagents examined--acetylacetone, ethyl acetoacetate, and methyl acetoacetate--the last was the best. When the ALA-pyrrole is reacted with Ehrlich's reagent in ethyl acetate according to one procedure, the optimum concentration of perc...

Although colorimetry (method of Shaw) permits neither a differentiation of epinephrine and norepinephrine nor their separation from other catechols, it reveals the total urinary catechol excre-tion, including those portions which were inactivated in the body. No significant aklnormalities were found in arterial hypertension, in congestive heart failure, after myocardial infarction and during em...

Urinary pyrrole, also known as the mauve factor (malvaria), pyrroluria and kryptopyrrole, was first described in the 1950s. It was found in the urine of patients undergoing experimental LSD treatments. When reacted with Ehrlich’s reagent (p-dimethylamino benzaldehyde), several of the urines developed a purple or “mauve” color. This colored compound was first thought to be an indole. In 1961, a ...

BACKGROUND It would be useful to develop a simple kit method for determination of thiocyanate in urine, which could be used to monitor cyanide overload in cassava-consuming populations. METHODS The method was based on the quantitative oxidation of thiocyanate in acid permanganate at room temperature in a closed vial with liberation of HCN, which reacted with a picrate paper. For semiquantitat...

OBJECTIVE To evaluate the possible relationship between the human tissue kallikrein amidase activity with sex and ethnicity in Brazilian primary hypertensive patients. DESIGN Population-based study. PARTICIPANTS One hundred men and women, Black and White primary hypertensive patients aged 20 years and older were selected. Eighty nine healthy individuals, paired according to age, sex, and et...

A simple test for the determination of cystinuria and of aminoaciduria is described. The basisof the test is the color reaction between nitroprussideand thiol groups. The reagent is a dry mixture to which the urine samplesare applied. The ease of peformance of the test makes it ideal for use as a screening procedure. The reaction is nonspecific, and positive tests require further investigation ...