Background: Sonication fluid culture is routinely conducted in patients with suspected orthopedic device-related infection (ORI). However, its value the assessment of devices that have explanted because presumed aseptic loosening still debatable. A meta-analysis was to estimate yield sonication detecting presence microorganisms loosening, and summarize clinical characteristics outcomes these pa...

In this paper, in addition to some elementary facts about the ultra-groups, which their structure based on the properties of the transversal of a subgroup of a group, we focus on the relation between a group and an ultra-group. It is verified that every group is an ultra-group, but the converse is not true generally. We present the conditions under which, for every normal subultra-group of an u...

A modified, quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction procedure combined with sonication Ultra-High Performance Liquid Chromatography–Orbitrap-Mass Spectrometry (UHPLC–Orbitrap-MS) was developed as a sensitive reliable methodology for the determination of multiclass pesticides in full-fat milk. Different amounts EMR-lipid sorbent were assayed cleanup step order to ac...

Uniformly-sized preparations with average microbubble (MB) diameters from 1 to 7 μm were produced reliably by sonicating decafluorobutane-saturated solutions of serum albumin and dextrose. Detailed protocols for producing and size-separating the MBs are presented, along with the effects that changing each production parameter (serum albumin concentration, sonication power, sonication time, etc....

Epigenetic studies increasingly require analysis of a small number of cells that are of one specific type and derived from patients or animals. In this report, we demonstrate a simple microfluidic device that integrates sonication and immunoprecipitation (IP) for epigenetic assays, such as chromatin immunoprecipitation (ChIP) and methylated DNA immunoprecipitation (MeDIP). By incorporating an u...

OBJECTIVES The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. METHODS A series of physical and chemical denaturation methods were implemented on well-defined 86-bp dsDNA fragment. The degree of each denaturation was measured and the most suitable denaturation method was de...

BACKGROUND The objective of this study is to present the results of cement spacer sonication in the second stage of two-stage treatment of shoulder arthroplasty infection and to determine the rate of positive cultures in the second-stage surgery in shoulder arthroplasty and its meaning. METHODS Twenty-one patients (22 cement spacers) treated with two-stage surgery because of a shoulder arthro...

Introduction: When treating periprosthetic joint infections with a two-stage procedure, antibiotic-impregnated spacers are used in the interval between removal of prosthesis and reimplantation. According to our experience, cultures of sonicated spacers are most often negative. The objective of our study was to investigate whether PCR analysis would improve the detection of bacteria in the space...

The diagnostic yield of prosthetic joint-associated infection is hampered by the phenotypic change of bacteria into a sessile and resistant form, also called biofilm. With sonication, adherent bacteria can be dislodged from the prosthesis. Species identification may be difficult because of their variations in phenotypic appearance and biochemical reaction. We have studied the phenotypic, genoty...

We have observed that hamster prion protein (PrP(C)) undergoes conformational changes on exposure to heat or sonication. If a sonication induced new conformer is seeded with a small amount of its abnormal pathogenic isoform (PrP(Sc)) it undergoes a significant conversion to a proteinase-resistant isoform. This suggests the presence of a third stable PrP conformer, which may be intermediate in t...