Competitive PCR is a frequently used technique for quantitation of DNA and mRNA. However, the application of the most favourable homologous mutated competitors is impeded by the formation of heteroduplex molecules which complicates the data evaluation and may lead to quantitation errors. Moreover, in most cases a single quantitation of an unknown sample requires multiple competitive reactions f...

background and purpose: microsporidiosis is one of the emerging and opportunistic infections, which causing various clinical symptoms in humans. the prevalence of this infection varies, depending on the infected organ, diagnostic methods, and geographical conditions. in the present study, we aimed to investigate microsporidial keratitis in patients referring to farabi eye hospital tehran, iran ...

background: the diagnosis of ocular toxoplasmosis is mainly based on clinical features. however, ocular fluid testing by pcr may be very helpful for approval or rejection of this etiology. in this study, we utilized a nested-pcr technique, targeting the b1 partial sequence to analyze the aqueous and vitreous samples for evaluating the presence of the toxoplasma dna. methods: fifty aqueous or vi...

background: cryptosporidiosis is a common coccidian parasite infection in patients with diarrhea that has worldwide distribution especially in developed countries. therefore,the aim of this study was to determine the occurrence of cryptosporidium infection in patients with gastroenteritis admitted to hospitals of mazandaran university of medical sciences by parasitological and molecular methods...