An automatic in vitro gas production technique was evaluated for predicting in vivo fiber (NDF) digestibility and effective first-order digestion rate of potentially digestible NDF (pdNDF) of 15 grass silages. Observed in vivo NDF digestibility of the silages harvested at different stages of maturity during 3 yr was determined by the total fecal collection in sheep fed at the maintenance level ...

With slight modification of a trypsin digestion technique, Rickettsia rickettsii were demonstrated specifically by immunofluorescence staining in Formalin-fixed, paraffin-embedded tissue sections from a human, rhesus monkey, and guinea pig with Rocky Mountain spotted fever and in infected membranes from a chicken embryo. Tissues were cut at 4 micron and, using geltain as a tissue adhesive, were...

We describe a simple and convenient method for processing small amounts of tissue samples for trace-metal measurements by atomic absorption spectrometry, by use of a modified Parr microwave digestion bomb. Digestion proceeds rapidly (less than or equal to 90 s) in a sealed Teflon-lined vessel that eliminates contamination or loss from volatilization. Small quantities of tissue (5-100 mg dry wei...

DNA is often subjected to multiple enzymatic treatments during subcloning procedures. For example, converting a restriction site to another by insertion of a synthetic linker requires five or six enzymatic steps: restriction enzyme digestion, treatment with DNA polymerase (if ends are not blunt), phosphorylation of linkers, ligation of linkers to blunt ends, removal of excess linkers by restric...

Introduction Echo-planar imaging (EPI) is a group of fast data acquisition methods commonly used in fMRI studies. It acquires multiple image lines in k-space after a single excitation, which leads to a very short scan time. A well-known problem with EPI is that it is more sensitive to distortions due to the used encoding scheme. Source of distortion is inhomogeneity in the static B0 field that ...

A technique for the extraction of cells from bone marrow trephine core biopsy specimens using collagenase digestion was assessed in 39 cases (33 diagnostic and six normal). Diagnostically useful numbers of cells were extracted from all marrows. Morphological assessment of cytocentrifuge preparations of these cells gave a correct diagnosis in 23 (60%) of cases compared with 27 (70%) for the corr...

Alfalfa, red clover, orchardgrass and timothy were harvested in the vegetative stage, wilted and stored as hay, or ensiled in small batch silos (20 kg) at 60, 40 or 20% (direct cut) dry matter and were analyzed for compositional differences. A ruminally cannulated lactating cow, consuming 50% of her dry matter intake from hay crop silage, was used to measure in situ dry matter, N, neutral deter...

Introduction Different barley varieties have been shown to affect in vitro dry-matter digestibility (IVDMD), ruminal starch digestion and animal performance (Boss and Bowman, 1996). Some of these differences may be due to differences in site and extent of nutrient digestion among barley varieties. Little information is available on digestive characteristics of different barley varieties and sev...

This paper investigates the issue of enabling scalable quantitative delay guarantee support in DiffServ networks through NSIS. A NSIS QoS Model is utilized to add the admission control framework to the DiffServ architecture and reservation-based admission control algorithms are designed for ingress and interior nodes respectively for enabling quantitative delay guarantees in a DiffServ domain. ...