the human papillomavirus l1 major capsid protein (hpv l1), the basis of the current vaccines, self-assembles into virus-like particles (vlps). herein, we describe the expression and purification of recombinant hpv16 l1 in e. coli system. the l1 protein was generated in a fused form using an inducible expression system. the recombinant gst-l1 fusion protein migrated as a 82 kda protein in sds-pa...

A novel DNA purification technology is described that enables the purification of pure dsDNA from blood. When compared to existing DNA purification methods, the Whatman BioScience Purification System (WBPS) offers a fast and convenient way to recover high yields of DNA. WBPS is based on a unique filter system that entraps DNA within a matrix. This allows the process to be performed in a single ...

Terrestrial ecosystems provide a number of vital services for people and society, such as biodiversity, food, fibre, water purification, carbon sequestration, and recreation. The future capability of ecosystems to provide these services is determined by changes in socio-economic trends, land use, biodiversity, atmospheric composition and climate. The primary objective of the project ATEAM (Adva...

This review focusses on affinity purification of immunoglobulins, a methodology which is a powerful tool to obtain pure and intact antibodies. Affinity techniques allow antibody purification both in a single step chromatographic procedure as well as in complex purification protocols depending on the intention to use the target antibody. The purification strategies for antibodies by interaction ...

montmorillonite is the major mineral of bentonite with many applications in industrial fields but some impurities decreases the quality of the bentonite. the main objective of this study was to investigate the suitable method for purification of ghaen mine bentonite. a combination of methods was considered including wet sieving and sedimentation, centrifuge and ultrasound. the efficiency of pur...

Joanne Beck, PhD, director of biologics manufacturing, Abbott Research Center Uwe Gottschalk, PhD, vice president, purification technology, Sartorius AG, Germany David W. Kahn, PhD, director, late-stage purification development, Human Genome Sciences, Inc. Duncan Low, PhD, scientific director, process development, Amgen Inc. Jill A Meyers, PhD, principal consultant, BioPro Consulting Charles Sc...

The biocompatibilities in blood purification therapy are defined as "a concept to stipulate safety of blood purification therapy by an index based on interaction in the body arising from blood purification therapy itself." The biocompatibilities are associated with not only materials to be used but also many factors such as sterilization method and eluted substance. It is often evaluated based ...

Background and Objective: The present study aimed to evaluate the optimum conditions for extracting and purifying penicillinase enzyme. The enzyme source was Bacillus licheniformis 6346 obtained from the Iranian Research Organization for Science and Technology. Materials and Methods: B. licheniformis was cultured in a medium containing mineral salts, nitrogen compounds, and cultured carbon sou...

Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed, namely; reverse micellar system, aqueous two phase...