Silver-intensified gold (SIG) particles were used for lightand electron-microscopic immunocytochemical localization of neuronal antigens, and the SIG method was compared with related heavy-metal methods for the purpose of dual ultrastructural localization of neurotransmitter-related antigens. SIG immunostaining was combined with peroxidase immunostaining to allow simultaneous study of different...

This study reports the in vitro cytotoxic effect of biologically synthesized silver and gold nanoparticles against MDA-MB-231, human breast cancer cells. Formation of silver and gold nanoparticles was observed within 30 min and the various characterization techniques such as UV-vis spectrophotometer, FE-SEM, TEM and XRD studies were confirmed the synthesis of nanoparticles. Further, MTT, acridi...

The staining of neurons with silver began in the 1800s, but until now the great resolving power of the laser scanning confocal microscope has not been utilized to capture the in-focus and three-dimensional cytoarchitecture of metal-impregnated cells. Here, we demonstrate how spectral confocal microscopy, typically reserved for fluorescent imaging, can be used to visualize metal-labeled tissues....

During a study of nucleolar organiser regions, a modified silver stain was found to be a sensitive marker for the iron in ringed sideroblasts, more so than Perls's stain when the marrow iron stores were low. To enhance the usefulness of the silver stain, a combined silver Perls method was developed. This stains the ringed sideroblast iron black and haemosiderin blue, thus rendering the detectio...

In this method for clinical measurement of /32-microglobulin, a 10 g/L agarose gel containing anti-/32-microglobulin is used, with subsequent staining with Coomassie Blue. Although the method is slow (requiring 30 h), it is inexpensive, reliable, and accurate over the range of ito 10 mg/L, and is useful in the determination of f32-microglobulin in serum and cerebrospinal fluid. A modified proce...

The capricious nature of silver impregnation techniques renders them unsuitable for bulk staining and screening sections for the presence of argyrophilic (senile) plaques and neurofibrillary tangles. For this purpose, we describe a simple, consistent and highly sensitive bulk staining method. It depends on the congophilia and resultant anomalous colour or polarization (Ladewig, 1945) that chara...

Rhodes' silver-plating technique for staining flagella was tested for its reliability and convenience as a routine procedure in the clinical laboratory. Modifications were made in the stain preparation and the procedure of staining and were tested with smears of known motile gram-negative nonfermentative bacilli. The stain has proved to be accurate and reliable and can be easily utilized with a...

The Golgi-Cox is a gold standard technique to stain the neurons. However the major limitations of the study are that it is time taking and inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were immersed in rapid Golgi solution and silver nitrate for staining of neurons in rat brain. The method is simple, reproducible, rapid, inexpensive a...

Dental caries is the most prevalent oral disease that continues to affect almost every country in world. The contemporary management of dental focuses on non-restorative, non-invasive, and micro-invasive treatment approaches arrest or reverse process at a lesion level reduce loss sound tooth structure. One these employment arresting agents possess antibacterial remineralizing properties. Silver...

In the present study, the combination of the micronucleus test with analysis of the activity of the rRNA genes in mutagen-treated Hordeum vulgare (barley) by maleic hydrazide (MH) cells was performed. Simultaneously fluorescence in situ hybridization (FISH) with 25S rDNA as probes and an analysis of the transcriptional activity of 35S rRNA genes with silver staining were performed. The results ...