A basic task of rescue robot systems is mapping of the environment. Localizing injured persons, guiding rescue workers and excavation equipment requires a precise 3D map of the environment. This paper presents a new 3D laser range finder and novel scan matching method for the robot Kurt3D [9]. Compared to previous machinery [12], the apex angle is enlarged to 360◦. The matching is based on sema...

Ribosome formation occurs in the nucleolus through interaction with various trans-acting factors. Therefore, hundreds of nucleolar proteins have a function ribosome formation, although precise each protein is largely unclear. We previously identified an uncharacterized protein, G-patch domain-containing 4 (GPATCH4 or G4), as component pre-ribosomes purified either nucleolin (NCL) NPM1. In this ...

Summary Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous factors. In this study, we analyze physiologic prokaryotic ribosome isolating bona fide pre-50S subunits from an Escherichia coli strain with factor ObgE, affinity tagged at its native gene lo...

Introduction : The process of protein synthesis is a vital for all kingdoms life. ribosome ribonucleoprotein complex that reads the genetic code, from messenger RNA (mRNA) to produce proteins and tightly regulate ensure cells growth. fact numerous diseases are caused by defect during biogenesis important understand this pathway. Materials methods We have analyzed literature its links with diffe...

Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It was previously shown that inserting an adenine residue at position 751 or intr...

During protein synthesis, tRNA and mRNA are translocated from the A to P to E sites of the ribosome thus enabling the ribosome to translate one codon of mRNA after the other. Ribosome translocation along mRNA is induced by the universally conserved ribosome GTPase, elongation factor G (EF-G) in bacteria and elongation factor 2 (EF-2) in eukaryotes. Recent structural and single-molecule studies ...

Ribosome profiling quantitatively captures ribosome locations during translation. The resulting profiles of ribosome locations are widely used to study translational speed. However, an accurate estimation of the ribosome location depends on identifying the A-site from ribosome profiling reads, a problem that was previously unsolved. Here, we propose a novel method to estimate the ribosome A-sit...