A novel template-dependent extension based isothermal amplification (TEIA) system with high single-base discrimination capability is developed, where the interference caused by non-specific reaction in isothermal strand displacement amplification (SDA) technique is substantially avoided via using a functionalized template probe, showing potential value in the development and application of SDA ...

Reaction intermediates formed during the degradation of duplex DNA by the recBC DNase have been characterized by sedimentation velocity, isopyknic centrifugation, electron microscopy, and susceptibility to various deoxyribonucleases which are specific for single-stranded DNA. It has been concluded that the intermediates are of two classes: (a) duplex molecules with single-stranded tails of seve...

A lateral flow biosensor for detection of single nucleotide polymorphism based on circular strand displacement reaction (CSDPR) has been developed. Taking advantage of high fidelity of T4 DNA ligase, signal amplification by CSDPR, and the optical properties of gold nanoparticles, this assay has reached a detection limit of 0.01 fM.

We have determined the mode of cleavage of superhelical SV40 DNA (Form I) by restriction endonucleases EcoRI and HpaII at 37 degrees C. By analysis with agarose gel electrophoresis and direct examination with dark field electron microscopy, we found that a large amount of the single-nicked circular DNA (Form II) was produced before the linear SV40 DNA (Form III) appeared. Thus, both restriction...

A Monte Carlo simulated annealing (MCSA) algorithm was used to generate the conformations of local regions in bovine pancreatic trypsin inhibitor (BPTI) starting from random initial conformations. In the approach explored, only the conformation of the segment is computed; the rest of the protein is fixed in the known native conformation. Rather than follow a single simulation exhaustively, comp...

Placental Lactogen is a polypeptide hormone that is produced by the Placenta, also known as chorionic somatomammotropin hormone. It has both Growth Hormone and Prolactin activities on growth, lactation, and luteal steroid production. The objective of this study was to investigate the bovine Placental Lactogen (bPL) gene polymorphism of Holstein cows in Razavi Khorasan province. Blood samples we...