We describe a method for determining uric acid in serum by reversed-phase liquid chromatography with spectrophotometric detection at 280 nm. Serum, 100 ul is mixed with 100 u1 of a solution containing, per liter, 70 ml of acetonitrile in sodium acetate (20 mmol/Iiter, pH 4.0) and 500 mg of the internal standard, adenine. The mixture is allowed to stand in an ice bath for 3 mm, then centrifuged....

The effect on enzyme kinetics of the presence of competitive inhibitors in constant molar ratio to t’he substrate has been described by Tubbs (I) and by Dalziel (2, 3). In this as yet unfamiliar situation, the inhibitor has the effect of increasing the apparent mutual affinity of enzyme and substrate. Thus, the slopes of reciprocal plots of the kinetic data are unchanged by the presence of the ...

A simple, direct, sensitive, and selective stopped-flow method for the fluorimetric determination of uric acid in serum and urine samples is described. The variation of fluorescence intensity during the reaction between uric acid and 1,1,3-tricyano-2-amino-1-propene (triap) in the presence of hydrogen peroxide is monitored. For these kinetic measurements peroxide is monitored. For these kinetic...

Uric acid in serum was determined calorimetrically with a batch type microcalorimeter, by measuring the heat evolved during a coupled uricase/catalase enzymic reaction in tris(hydroxymethyl)aminomethane HCl buffer (pH 9.0 at 30 degrees C). Heat evolution and concentration are linearly related through the physiological range of serum uric acid concentrations and the method is free of interferenc...

After several decades of senescence, the twin fields of hyperuricemia and gout have again regained attention in both the scientific and clinical spheres, and this review highlights several recent advancements. Specifically, we review newly discovered mechanisms of uric acid-induced inflammation, uric acid's putative role as a "danger signal" in innate immunity, the possible link between hyperur...

Treatment-failure gout (TFG) affects approximately 50,000 patients or about 1% of the overall population of patients with gout in the United States of America. The severity of TFG is manifested by frequent acute attacks of disabling arthritis, chronic deforming joint disease, destructive masses of urate crystals (tophi), progressive physical disability, and poor health-related quality of life. ...

1. On the basis of its action on a number of conjugated hexuronic acids and other compounds, it is concluded that mouse-liver glucuronidase is specific for f-D-glucopyranuronides, including ,B-D-glucopyranuronic acid-1-phosphate. 2. Derivatives of klevomenthol caused slight activation of glucuronidase. 3. Mouse-liver preparations did not catalyse the condensation of o-aminophenol with aor ,Bglu...

In 1939 Blnuch and Koch (1) published an enzymatic method for the calorimetric determination of uric acid in whole blood. This method involves the use of the enzyme uricase for the specific destruction of uric acid. “True uric acid” values were obtained by the difference in the intensity of color produced before and after incubation with the enzyme. The authors reported “true uric acid” values ...

OBJECTIVE To determine the current prevalence of hyperuricaemia and gout in New Zealand Maori and Europeans for comparison with previous studies. METHODS 342 Maori and 315 European men and women aged 15 years and older were studied by personal interview and a musculoskeletal system examination. The 1977 ARA criteria for gout in a survey setting were used and serum uric acid was determined by ...