the sip gene encoding for a conserved highly immunogenic surface protein of streptococcus agalactiae was amplified using polymerase chain reaction (pcr) and subcloned into prokaryotic expression vector pet32a (+) and expressed as a recombinant protein in e. coli bl21 (de3). an indirect enzyme linked immunosorbent assay (elisa) was developed using the purified sip protein as a coating antigen, w...

conclusions our results showed that the recombinant protein produced by the pbad vector in the escherichia coli system was very efficient. background nontypeable haemophilus influenzae (nthi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. protein d (pd) belongs to the minor outer-membrane proteins of h. in...

BACKGROUND The use of Salmonella to deliver heterologous antigens from DNA vaccines is a well-accepted extension of the success of oral Salmonella vaccines in animal models. Attenuated S. typhimurium and S. typhi strains are safe and efficacious, and their use to deliver DNA vaccines combines the advantages of both vaccine approaches, while complementing the limitations of each technology. An i...

BACKGROUND The dust mite Lepidoglyphus destructor is a major cause of allergic diseases among farmers. We have previously cloned and sequenced two isoforms of the major allergen Lep d 2 (formerly designated Lep d 1) and found significant homology to group 2 allergens of the house dust mite species Dermatophagoides. We now report on the production and characterization of recombinant Lep d 2. O...

Our approach in predicting gene expression levels relates to codon usage differences among gene classes. In prokaryotic genomes, genes that deviate strongly in codon usage from the average gene but are sufficiently similar in codon usage to ribosomal protein genes, to translation and transcription processing factors, and to chaperone-degradation proteins are predicted highly expressed (PHX). By...

background: recombinant vaccine technology is one of the most developed means in controlling infectious diseases. however, an effective vaccine against shigella is still missing. objective: to evaluate recombinant ipac protein of shigella as a vaccine candidate. methods: in this study we cloned ipac gene into an expression vector in prokaryotic system. the protein expression was evaluated by sd...

Low protein solubility and inclusion body formation represent big challenges in production of recombinant proteins in Escherichia coli. We have recently reported functional expression of hydroxynitrile lyase from Manihot esculenta, MeHNL, in E. coli with high in vivo solubility and activity using directed evolution. As a part of attempts to clarify the mechanism of this phenomenon, we have desc...

BACKGROUND The ability to produce the same recombinant protein in both prokaryotic and eukaryotic cells offers many experimental opportunities. However, the cloning of the same gene into multiple plasmids is required, which is time consuming, laborious and still may not produce soluble, stable protein in sufficient quantities. We have developed a set of expression vectors that allows for ligati...