Agrobacterium-mediated plant transformation has been used widely, but there are plants that are recalcitrant to this type of transformation. This transformation method uses bacterial strains harboring a modified tumor-inducing (Ti) plasmid that lacks the transfer DNA (T-DNA) region (disarmed Ti plasmid). It is desirable to develop strains that can broaden the host range. A large number of Agrob...

We have efficiently amplified plasmid DNA from single yeast colonies using rolling circle amplification (RCA). The amplified DNA can be directly used for restriction digestion, DNA sequencing, or yeast transformation. The RCA-based high-fidelity amplification would be useful for plasmid manipulation in a variety of yeast-based systems, particularly for high-throughput analyses.

No recombination between plasmids was observed after conjugal transfer of a plasmid into a cell carrying another plasmid. Two types of such recombination took place after transformation, one type being Rec+ dependent and suggesting a preferred site of recombination. The other much rarer type was at least partially Rec+ independent.