نتایج جستجو برای: pcr typing
تعداد نتایج: 193438 فیلتر نتایج به سال:
Background & Aims: The efflux pumps play an important role in the development of drug resistance in Klebsiella pneumoniae. The aim of this study was to investigate the antibiotic resistance, and the presence of efflux genes, as well as molecular typing in clinical isolates of Klebsiella pneumoniae. Materials & Methods: In this cross sectional descriptive study, a total of 100 K. pneumoniae iso...
This study examined Shiga toxin-producing Escherichia coli (STEC) O157, using phage typing, pulsed-field gel electrophoresis, and typing of Shiga toxin variant genes by PCR with restriction fragment length polymorphism in an epidemiological survey of STEC O157 isolated from humans in Finland between 1990 and 1999.
Methods The usefulness of spa-typing, pulse-field gel electrophoresis (PFGE) and automated repetitive extragenic palindromic sequence-based (rep-PCR) method (DiversiLab, Bacterial Barcodes, Inc.) for typing Finnish MRSA strains was studied. A total of 110 clinical MRSA isolates collected from well-defined outbreaks in Ostrobothnia region and 96 isolates from several epidemics or sporadic source...
The sequencing of the VP1 hypervariable region of the human enterovirus (HEV) genome has become the reference test for typing field isolates. This study describes a new strategy for typing HEV at the serotype level that uses a reverse transcription-PCR assay targeting the central part of the VP2 capsid protein. Two pairs of primers were used to amplify a fragment of 584 bp (with reference to th...
Large-scale pharmacogenetics and complex disease association studies will require typing of thousands of single-nucleotide polymorphisms (SNPs) in thousands of individuals. Such projects would benefit from a genotyping system with accuracy >99% and a failure rate <5% on a simple, reliable, and flexible platform. However, such a system is not yet available for routine laboratory use. We have eva...
164 Abstract We have developed an allotyping assay for detection of four HLA DRB1*01 group alleles based on polymerase chain reaction and solution hybridization in a microtiter plate. Using group specific primers a region within exon 2 of HLA DRB1 gene was amplified by PCR. Labeling of PCR product was achieved by adding small amount of Dig-dUTP in place of dTTP. Labeled PCR product was hybridiz...
In this study, we evaluated three PCR-based methods for the molecular typing of nonpathogenic Fusarium oxysporum isolates: random amplified polymorphic DNA (RAPD), polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and amplified fragment length polymorphism (AFLP). The analyses were performed using 64 isolates of F. oxysporum collected from cotton-producing areas in E...
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