Nitric oxide is a short-lived mediator, which can be induced in a variety of cell types and produces many physiologic and metabolic changes in target cells. It is important in many biological functions and generated from L-arginine by the enzyme nitric oxide synthase. Nitric oxide conveys a variety of messages between cells, including signals for vasorelaxation, neurotransmission and cytotoxici...

Purified preparations of rabbit skeletal muscle glycogen synthase (specific activity 6 to 42 units/mg of protein) were found to be contaminated with calciumdependent kinase(s) which catalyzed the phosphorylation of glycogen phosphorylase b and glycogen synthase. In a series of glycogen synthase preparations there was a correlation between the phosphorylation rates of glycogen synthase and of ad...

Ceramides, which derive from the hydrolysis of glucosylceramide (GlcCer), are the predominant lipid species in the stratum corneum and are critical for epidermal permeability barrier homeostasis. UDP-glucose:ceramide glucosyltransferase (GlcCer synthase) (EC 2.4.1.80) catalyzes the glucosylation of ceramide to form GlcCer. Recently, we demonstrated a progressive increase in GlcCer synthase expr...

Diethylstilbestrol (DES) has been found to be oxidized in Syrian hamster embryo (SHE) cells by prostaglandin-H synthase (PGH synthase). It is hypothesized that PGH synthase mediates adverse effects of DES and other carcinogenic estrogens such as induction of neoplastic transformation and genotoxicity. Interest in PGH synthase-catalyzed reactions focuses on two aspects: oxidation and metabolic a...

The rise in the resistant strains has ignited investigation of ability these essential oils to have an effect against them. aim this study was investigate antibacterial activity clove oil, oregano oil and tea tree Escherichia coli patient isolate Acinetobacter baumannii. Ciprofloxacin used as standard DMSO at 1 % negative control. To understand inhibitory action on ATP synthase, docking analysi...

Sugar nucleotide–modifying enzymes of the short-chain dehydrogenase/reductase type use transient oxidation–reduction by a tightly bound nicotinamide cofactor as common strategy catalysis to promote diverse set reactions, including decarboxylation, single- or double-site epimerization, and dehydration. Although basic mechanistic principles have been worked out decades ago, finely tuned control r...