BACKGROUND Media containing yeast extracts and other complex raw materials are widely used for the cultivation of microorganisms. However, variations in the specific nutrient composition can occur, due to differences in the complex raw material ingredients and in the production of these components. These lot-to-lot variations can affect growth rate, product yield and product quality in laborato...

Our present work focuses on the coupling between thermal diffusion and convection in order to improve the thermal gravitational separation of mixture components. The separation phenomenon was studied in a porous medium contained in vertical columns. We performed analytical and numerical simulations to corroborate the experimental measurements of the thermal diffusion coefficients of ternary mix...

We study the effect of homogenization on flame propagation in periodic excitable media, when the width of the flame is much smaller than the characteristic size of the heterogeneities.

In this paper, we have attempted optimization of production of enzyme carboxymethylcellulase or endoglucanase from the bacterium Bacillus amyloliquefaciens SS35. Optimization has been carried out in two stages using statistical experimental design, viz. medium optimization and optimization of fermentation parameters. For medium optimization, Plackett-Burman design followed by central composite ...

Aims. We present new radio continuum observations of ten BIMA SONG galaxies, taken at 1.4 GHz with the Very Large Array. These observations allow us to extend the study of the relationships between the radio continuum (RC) and CO emission to 22 CO luminous galaxies for which single dish CO images have been added to interferometric data. New Spitzer infrared (IR) images of six of these galaxies ...

Lactase has excellent applications in dairy industry and commercially this enzyme is produced from bacterial sources but not in high yields. In this work, the production of lactase was improved by designing of nutrient components in fermentation medium by one factor at a time. Lactose and yeast extract were selected as preferable carbon and nitrogen sources for lactase production with tryptopha...

Introduction A series of cell-line specific complex media supplements have been developed for enhancing performance of various vaccine production systems. Created using in-house media optimization methods, these supplements are manufactured using innovative process technology which allows for the formulation of complex and/or chemically defined animal-component free media additives into a singl...

The use of chemically defined culture media for production of biopharmaceuticals has become state of the art. In combination with a suitable feed solution chemically defined media are the most potent driving factor for yield improvement in fed-batch processes. Although various chemically defined media are available of-the-shelf and deliver good results, those media are developed with the aim to...

Although the pathways by which propionibacteria ferment glucose have not been completely established, it has been noted by several investigators that relatively large numbers of bacterial cells are required for reasonable reaction rates. During studies on glucose fermentationi by intact cells of Propionibacterium freudenreichii we have found that the addition of the medium components described ...

In recent years there has been increasing interest in the use of polarization for imaging objects in a cluttered environment. Examples are optical imaging through clouds, optical detection of objects in a biological medium, and microwave detection of objects in clutter. We extend previous studies of continuous-wave scattering to pulse-polarization scattering in discrete scatterers. We solve the...