The efficient partitioning of the 2m m plasmid of Saccharomyces cerevisiae at cell division is dependent on two plasmid-encoded proteins (Rep1p and Rep2p), together with the cis-acting locus REP3 ( STB ). In addition, host encoded factors are likely to contribute to plasmid segregation. Direct observation of a 2m m–derived plasmid in live yeast cells indicates that the multiple plasmid copies a...

BACKGROUND As the supply of starch grain and sugar cane, currently the main feedstocks for bioethanol production, become limited, lignocelluloses will be sought as alternative materials for bioethanol production. Production of cellulosic ethanol is still cost-inefficient because of the low final ethanol concentration and the addition of nutrients. We report the use of simultaneous saccharificat...

Sterile-alpha-motif (SAM) domains are common protein interaction motifs observed in organisms as diverse as yeast and human. They play a role in protein homo- and hetero-interactions in processes ranging from signal transduction to RNA binding. In addition, mutations in SAM domain and SAM-mediated oligomers have been linked to several diseases. To date, the observation of heterogeneous SAM-medi...

conclusions our results demonstrated no significant correlation between the ird5 lifespan and the trehalose concentration. likewise, the correlation between lifespan extension, trehalose accumulation, and cellular resistance to hydrogen peroxide was not significant. results the lifespan of the ird5 mutant was significantly longer that of either the ird11 mutant or the wild type cells. under rep...

Exact law of mortality dynamics in changing populations and environment is derived. The law is universal for all species, from single cell yeast to humans. It includes no characteristics of animal-environment interactions (metabolism etc) which are a must for life. Such law is unique for live systems with their homeostatic self-adjustment to environment. Its universal dynamics for all animals, ...

Introduction Breweries, such as those operated by Anheuser Busch, Inc., utilize the Coulter Principle for their yeast cell enumeration and sizing. This technology uses the ZTM Series instrument models and the Multisizer 3. This document will focus on the Z Series models: the Z1 Single Threshold, the Z1 Dual Threshold, and the Z2 Analyzer. Yeast cells are analyzed during the fermentation process...

Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. The concept of individual microorganisms influencing the makeup of T cell subsets via interactions with intestinal dendritic cells (DCs) appears to constitute the foundation for immunoregulatory effects of probiotics, and several studies have reported probiotic strains resulting i...