BACKGROUND We previously cloned a 1,3-specific lipase gene from the fungus Rhizomucor miehei and expressed it in methylotrophic yeast Pichia pastoris strain GS115. The enzyme produced (termed RML) was able to catalyze methanolysis of soybean oil and showed strong position specificity. However, the enzyme activity and amount of enzyme produced were not adequate for industrial application. Our go...

The gut regions of Epilachna chrysomelina are endowed with multiple enzymes that aid digestion host plant tissues. Digestive present in the E. during various developmental stages were studied laboratory to determine most destructive life stage beetle for proper management measures. Cellulase, α-glucosidase, amylase, lipase and proteinase activities observed guts. mid-gut recorded significantly ...

Lipophilic esters of hydroxytyrosol have been synthesized in a one-pot procedure from tyrosol and long side-chain carboxylic acids by lignin nanoparticles supported enzymatic cascade lipase M tyrosinase. The reaction involved the initial esterification aliphatic group with hydroxyl followed ortho-hydroxylation aromatic moiety. Lipase tyrosinase were immobilized on layer-by-layer using chitosan ...

Candida rugosa lipase (CRL) was deposited by matrix assisted pulsed laser evaporation (MAPLE) in order to immobilize the enzyme with a preserved native conformation, which ensures its catalytic functionality. For this purpose, the composition of the MAPLE target was optimized by adding the oil phase pentane to a water solution of the amino acid 3-(3,4-dihydroxyphenyl)-2-methyl-l-alanine (m-DOPA...

Fats and oils as major dietary components are involved in the development of chronic diseases. In this paper the physiological relevance and some methodological aspects related to the determination of two enzymes enrolled in metabolism of fat – pancreatic lipase and arylesterase – are discussed. Pancreatic lipase has been extensively used to study the triacylglycerol fatty acid composition and ...

The lipoprotein lipase (clearing-factor lipase) activity of the white adipose tissue from rats aged between 1 and 145 days was determined. Five adipose-tissue sites (epididymal, uterine, subcutaneous, perirenal and intramuscular) together with serum concentrations of triacylglycerol, cholesterol and glucose were studied. The pattern of enzyme-activity change was remarkably similar in all the si...

Lipase from Aspergillus niger was “doubly immobilized” with SiO2 nanoparticles in sol-gel powders prepared via the base-catalyzed polymerization of tetramethoxysilane (TMOS) and methyltreimethoxysilane (MTMS). The hydrolytic activity of the immobilized lipase was measured using the p-nitrophenyl palmitate hydrolysis method. The results showed that the optimum preparation conditions for the gels...

A triacylglycerol lipase, presumably the first enzyme involved in the mobilization of lipid from the insect fat body, has been purified to homogeneity from the fat body of Manduca sexta. The purification procedure involved polyethyleneglycol precipitation, and chromatography on DEAE-cellulose, phenyl-Sepharose, Q-Sepharose and hydroxylapatite. The final product, a protein with an M(r) = 76,000 ...

The study is attempted to investigate the production of potent lipase from groundnut oil mill fungi. The fungi was isolated and identified as Trichoderma sp. Lipolytic activity of Trichoderma sp. was preliminarily confirmed by Rhodamine B method, lipase production was carried out using mineral medium. The extracellular protein concentration and lipase enzyme activity (7.83U/ml) was maximum at 4...