background and objectives: bitter melon is one of the anti-cancer medicinal plants that it’s wide level in vitro propagation and anti-cancer material production was necessary. different factors such as genotype, selected explants’ type and it`s preparation conditions influenced regeneration content. plant growth regulator`s effect on callogenesis and indirect regeneration from parangipetai indi...

tissue culture is an attractive alternative for plant propagation. micropropagation is a technique to ensure a constant and uniform source of ornamental plants. matthiola incana is an important ornamental species mainly cultivate by seed. matthiola incana seeds were germinated on solid ms medium without plant growth regulators. shoot proliferation and root formation are possible using kinetin (...

Durian lahung is a type of endemic durian to Kalimantan which has unique characteristic, namely its red skin. The existence this plant almost extinct so that conservation needs be done and one way use tissue culture techniques. As first step, it necessary conduct experiments obtain the right sterilization method in order aseptic planting material. This study aims determine effectiveness sterila...

lemon balm (melissa officinalis l.) is an herbaceous perennial plant of the lamiaceae family that has a wide edible and medicinal uses. in this research the potential of five different explants (nodal segments, shoot tip, cotyledon, hypocotyl and leaf segments) on ms mediums supplemented with different concentrations of 6-benzylaminopurine (bap) (2.2, 4.4 and 8.8 µm) alone or in combination wit...

The present study was carried out to evaluate the effects of light and plant growth regulators on callus induction and explant browning in date palm cultivar, Estameran in in vitro condition. The explants were pretreated by an antioxidant combination (100 mgl-1 citric acid and 150 mgl-1 ascorbic acid) for 3 days in 5 °C. Then, the  leaf explants  were cultured in Murashige and Skoog medium (MS)...

Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paro...

Ficus lyrata is a popular ornamental foliage plant with unique violin- or guitar-shaped green leaves. In our previous study, grapevine gene VvMybA1 was introduced into F. via Agrobacterium-mediated transformation, which resulted in the availability of purple-leaved plants. Since transcription factor, regeneration transgenic plants might potentially produce variants multicolored The objective th...