The ability of prostaglandin El (PGE1) and cholera toxin to increase cyclic AMP levels is potentiated g-fold when normal rat kidney (NRK) cells are treated with picolinic acid or histidinol, or grown in isoleucine-deficient medium. The response to (-)-isoproterenol is increased a-fold in NRK cells treated with picolinic acid but not in cells subjected to isoleucine deprivation. The increase in ...

The circular dichroic spectra of oxytocin and four analogues of oxytocin are presented at several pH values in the wave length interval, 220 to 320 rnp. The analogues are 2-isoleucineand 2-glycine-oxytocin, in which the tyrosine residues in Position 2 have been replaced by isoleucine and glycine, respectively, as well as deamino-oxytocin and deamino-2-isoleucine-oxytocin, in which the a-NH2 gro...

Addition of L-valine (50 to 200 mM) to glucose minimal medium had no effect on the growth of wild-type Corynebacterium glutamicum ATCC 13032 but inhibited the growth of the derived valine production strain VAL1 [13032 DeltailvA DeltapanBC(pJC1ilvBNCD)] in a concentration-dependent manner. In order to explore this strain-specific valine effect, genomewide expression profiling was performed using...

BACKGROUND Amino acid assimilation is crucial for bacteria and this is particularly true for Lactic Acid Bacteria (LAB) that are generally auxotroph for amino acids. The global response of the LAB model Lactococcus lactis ssp. lactis was characterized during progressive isoleucine starvation in batch culture using a chemically defined medium in which isoleucine concentration was fixed so as to ...

BACKGROUND Amino acid dehydrogenases (L-amino acid: oxidoreductase deaminating; EC 1.4.1.X) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either NAD+, NADP+ or FAD. These enzymes have been received much attention as biocatalysts for use in biosensors or...

The influence of the relative concentrations of amino acids in culture media on the growth of microorganisms was first brought to the attention of microbiologists by Gladstone (1939). He observed that isoleucine, valine, or leucine added singly to a synthetic medium inhibited the growth of the anthrax bacillus whereas a mixture of the three stimulated growth. Although Gladstone was unable at th...

Aim . Branched-chain amino acids (BCAAs) have been postulated as potential indicators of cardiovascular risk. The objective this study was to explore the relationship between plasma BCAAs and different stages disorders. Material methods In our cross-sectional study, (valine, leucine isoleucine) in individuals without diseases (CVDs) (nonCVD group, total n=27, with n=16 healthy, but metabolic di...

BACKGROUND The proteinogenic branched-chain amino acids (BCAAs) valine, leucine and isoleucine might play an unrecognised crucial role in the development of depression through their activation of the mammalian target of rapamycin (mTor) pathway. The aim of this research project is to evaluate whether BCAAs are altered in patients with major depression and might thus be appropriate biomarkers fo...