Mitochondria were isolated from autotrophically grown Chlamydomonas reinhardtii cell-wall-less mutant CW 92. The cells were broken by vortexing with glass beads, and the mitochondria were collected by differential centrifugation and purified on a Percoll gradient. The isolated mitochondria oxidized malate, pyruvate, succinate, NADH, and [alpha]-ketoglutarate. Respiratory control was obtained wi...

Ghiretti, F. & Libonati, M. (1957). Acta Embryol. Morphol. Exper. 1, 48. Ghiretti-Magaldi, A., Giuditta, A. & Ghiretti, F. (1957). Pubbl. Staz. zool. Napoli (in the Press.) Keilin, D. (1925). Proc. Roy. Soc. B, 98, 312. Keilin, D. & Hartree, E. F. (1937). Proc. Roy. Soc. B, 122, 298. Keilin. D. & Hartree, E. F. (1939). Proc. Roy. Soc. B, 127, 167. Loewus, F. A., Westheimer, F. H. & Vennesland, ...

A comparison was conducted of current methods for estimation of the activity states (proportion of enzyme in active, dephosphorylated, form) of hepatic branched-chain 2-oxo acid dehydrogenase. Practically all of the enzyme was active in freeze-clamped liver obtained from chow-fed and 48 h-starved rats, regardless of the presence of fluoride in the extraction and assay media to inhibit phosphata...

It seems likely that the mechanism of mitochondrial oxidative phosphorylation will be understood only after the components of the system have been separated and identified, and the mechanism of their action examined in detail. Experiments in this laboratory, therefore, have been directed toward the resolution of the oxidative phosphorylation system of beef heart mitochondria into its component ...

Skeletal muscle mitochondria are arranged as a reticulum. Insight into the functional characteristics of such structure is achieved by viewing the network as consisting of "subsarcolemmal" (SS) and "intermyofibrillar" (IMF) regions. During the decades, most, but not all, published studies have reported higher (sometimes over 2-fold) enzyme and enzyme-pathway protein-specific activities in IMF c...