Background: In Algeria, date palm is currently confronted to the Bayoud disease. Biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species. Objectives: Callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars. Materials and Methods: Some factors influencing the isolatio...

OBJECTIVE To determine the frequency and compare the culture yield of bacterial isolation by conventional and blood culture BACTEC bottle techniques in cirrhotic patients with spontaneous bacterial peritonitis (SBP). STUDY DESIGN Cross-sectional comparative study. PLACE AND DURATION OF STUDY Pathology Department, Bannu Medical College, Bannu, KPK, from January 2012 to December 2013. METHO...

Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...

mycoplasma agalactiae (m. agalactiae) is one of the main causes of contagious agalactia, an infectious syndrome of sheep and goats in khuzestan province –southwest of iran that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. this study was carried out to isolation and identification of m. agalactiae with culture and polymeras...

contagious agalactia (c.a) is an infectious syndrome of sheep that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. mycoplasma agalactiae (m. agalactiae) is the main cause of the disease in sheep. the aim of this study was isolation and identification of m. agalactiae with culture and polymerase chain reaction (pcr) assay f...

History of Plant Tissue Culture and Biotechnology Scope and Importance of Biotechnology Tissue and Cell Culture Micropropagation Anther Culture Production of Secondary Metabolites Protoplasts Isolation and Fusion Somatic Hybrids Somaclonal Variation Germplasm Conservation Genetic Engineering and Gene Technology Methods for Gene Transfer Generation of Transgenic Plants and their identification M...

Human bone marrow derived mesenchymal stem cells (BM-MSCs) resides in their niches in close proximity to hematopoietic stem cells (HSCs). These naïve MSCs have tremendous potential in regenerative therapeutics, and may also be exploited by cancer and infectious disease agents. Hence, it is important to study the physiological and pathological roles of naïve MSC. However, our knowledge of naïve ...

During isolation of Venturia inaequalis (Cooke) G. Winter from apple leaves and fruits in the laboratory, some fungi appeared as culture contaminants. From these, an isolate was identified as Aspergillus medius R. Meissn. (teleomorph known as Eurotium medium C.F.W. Meissn.), based on morphological and molecular data (sequences of ITS rDNA).

during isolation of venturia inaequalis (cooke) g. winter from apple leaves and fruits in the laboratory, some fungi appeared as culture contaminants. from these, an isolate was identified as aspergillus medius r. meissn. (teleomorph known as eurotium medium c.f.w. meissn.), based on morphological and molecular data (sequences of its rdna).