A Leukemia-Associated CD34/CD123/CD25/CD99-positive Immunophenotype Identifies FLT3-mutated Clones in Acute Myeloid Leukemia Daniela F. Angelini*, Tiziana Ottone,* Gisella Guerrera, Serena Lavorgna, Michela Cittadini, Francesco Buccisano, Marco De Bardi, Francesca Gargano, Luca Maurillo, Mariadomenica Divona, Nélida I. Noguera, Maria Irno Consalvo, Giovanna Borsellino, Giorgio Bernardi, Sergio ...

PURPOSE The transcription factor CCAAT/enhancer binding protein alpha, encoded by the CEBPA, is crucial for the differentiation of immature granulocytes. Mutation of the CEBPA may play an important role in leukemogenesis and prognosis. We sought to characterize the CEBPA mutation in acute myeloid leukemia (AML) and to clarify if there is a distinct immunophenotype for leukemic cells with the mu...

BACKGROUND A T-cell clone, thought to be the source of eosinophilopoietic cytokines, identified by clonal rearrangement of the T-cell receptor and by the presence of aberrant T-cell immunophenotype in peripheral blood defines lymphocytic variant of hypereosinophilic syndrome (L-HES). DESIGN AND METHODS Peripheral blood samples from 42 patients who satisfied the diagnostic criteria for HES wer...

BACKGROUND Primary intestinal NK/T cell lymphoma is extremely rare and early diagnosis is frequently difficult. The aim of this study is to investigate the clinicopathological findings, immunophenotype, and T cell receptor (TCR) γ gene rearrangement of primary intestinal NK/T cell lymphomas in 25 Chinese cases. METHODS Clinical data of the 25 cases were analyzed. Immunohistochemistry for immu...

The coexistence of large granular lymphocytic leukemia (LGLL) and pure red cell aplasia (PRCA) has been previously described, but is rare in Western countries (7% in a recent series of LGLL cases). We present the clinical features, hematological parameters and immunophenotype of two patients with PRCA associated with CD3+ LGLL.

Cytogenetics study using combination of conventional cytogenetics and fluorescent insitu hybridization was carried out in 171 pediatric acute lymphoblastic leukemia patients subgrouped to B-ALL (n=126) and T-ALL (n=45) by bone marrow morphology and immunophenotype. The chromosomal aberration frequency in B-ALL and T-ALL was 79% and 71%, respectively. TEL/AML1 translocation was detected in 28% o...

A 56-year-old woman presented with diffuse xerotic cutaneous plaques and lymphadenopathy. Her peripheral count showed marked lymphocytosis (white cell 31.8 × 109/L), mild anemia (hemoglobin 10.7 g/dl), thrombocytosis (platelet 483 109/L). blood smear demonstrated numerous small to medium-sized lymphocytes significantly irregular nuclei, delicately folded/cerebriform nuclei (Sézary cells) (Image...

Diffuse large B-cell lymphoma (DLBCL) is the term applied to a constellation of different disorders with specific clinicopathological features [1]. All DLBCL cases are distinguished by a diffuse pattern of infiltration and a cytological composition by large Bcells. Despite these common features, DLBCL cases may have strikingly different clinical presentations, immunophenotype, molecular pathoge...

Alveolar soft part sarcoma (ASPS) is a tumor of unknown histogenesis, composed of large, epithelioid cells with eosinophilic cytoplasm, having an alveolar pattern. Primary ASPS of uterine cervix is very rare. In this report, we present a 21-aged-old female with primary ASPS in the uterine cervix and discuss the clinicopathological characteristics, immunophenotype, molecular genetic feature and ...

10.1128/CDLI.7.3.352-359.2000. 2000, 7(3):352. DOI: Clin. Diagn. Lab. Immunol. Immunology Advanced Technology Laboratories Christine E. Nickerson, Alan L. Landay and The ACTG Keith A. Reimann, Miriam Chernoff, Cynthia L. Wilkening, Clinical Trials Donors: Implications for Multicenter Immunodeficiency Virus Type 1-Infected Blood Mononuclear Cells from Human Responses in Cryopreserved Peripheral ...