SAKURADA, T., SAITO, S., YAMAGUCHI, T., YAMAMOTO , M., DEMURA, R., DEMURA, H., FUKUGHI, S., YOSHIDA, K. and TORIKAI, T. Radioinonunoassay of Triiodothyronine. Tohoku J. exp. Med ., 1973, 110 (4), 329-340 Immuniza tion of rabbits with triiodothyronine-methyl ester hydrochloride conjugated to bovine serum albumin resulted in the production of antibody against triiodothyro nine. Using dextran-coat...

objective: the prevalence of respiratory allergies, especially those induced by fungi such as alternaria alternata, has dramatically increased over the past decade. this increase has caused major health problems worldwide. this study aimed to investigate the role of a. alternata in the etiology of allergic asthma, by using the skin prick test and assessment of ige specific to the fungus in the ...

An assay technique, homogeneous enzyme immunoassay, is described for the quantitative determination of morphine derivatives in biological fluids. An enzyme is labeled with morphine. When the enzymelabeled morphine is bound by antimorphine antibodies, the enzyme is rendered inactive. Free morphine competes with enzyme-morphine for antibody binding sites preventing inhibition of enzyme activity. ...

OBJECTIVEdIn diabetic foot ulcers, wound fluid inflammatorymediators have previously been proposed as surrogate markers for nonhealing. However, currently available wound fluid sampling techniques are not suitable for clinical practice due to low levels of exudate and a high logistical effort. The aim of this investigation was to assess 1) the technique of superficial wound swabbing for harvest...

An immunoglobulin M (IgM) capture enzyme immunoassay technique was adapted for the detection of antibody to Colorado tick fever virus in sera from 84 individuals for whom diagnosis had been confirmed by virus isolation or neutralization test. Titers were compared with those for IgG and neutralizing antibodies in these Colorado tick fever cases. IgM antibody titers were higher than neutralizing ...

Results for abused drugs in urine, as obtained by radioimmunoassay (RIA), the “Enzyme Multiplied Immunoassay Technique” (“EM IT”), and hemagglutination inhibition (HI), were compared with each other, with fluorimetry, and with thin-layer chromatography (TLC). The immunoassays and fluorimetric methods were highly sensitive (30 tg/liter to 2 mg/ liter). Cross-reactivity (lack of specificity) vari...

A sensitive assay for the simultaneous detection of anti-cytomegalovirus and anti-herpes simplex virus antibodies was developed. Two different sizes of polystyrene microspheres were coated with purified viral antigens. Human antiviral antibodies were detected with a biotin-streptavidin amplification procedure with phycoerythrin as the fluorescent label. Microsphere-associated fluorescence was q...

Gentamicin was determined in serum by the homogeneous enzyme immunoassay system (EMIT) adapted to the Multistat centrifugal analyzer. The standard curve can be extended to 16 mg/L; however, poor precision is obtained at concentrations greater than 10 mg/L because a small difference in change in absorbance there will produce a significant variation in gentamicin concentration. For example, the w...

Immunoassay for low molecular weight food contaminants, such as pesticides, veterinary drugs, and mycotoxins is now a well-established technique which meets the demand for a rapid, reliable, and cost-effective analytical method. However, due to limited understanding of the molecular structure of antibody binding sites and antigenic epitopes, as well as the intermolecular binding forces that com...