Enzymes Immobilization onto different types of the substrate could be helpful in various applicationsof biomedical devices and biosensors. Enzyme activity and stability could be affected by support andmethod of immobilization. In this study, the silver metal foam was successfully synthesized by thesoft-shell method and then was coated with silica. Then, glucose oxidase (GOx) immobilized on nonc...

A novel fusion protein has been rationally designed, combining the hexameric glutamate dehydrogenase from Clostridium symbiosum with dimeric formate Candida boidinii . The former enzyme consumes ammonia for reductive amination of α-ketoglutarate using NADH, while latter biocatalyst regenerates continuously cofactor. This enzymes opens new perspectives detection and removal ammonia. bifunctional...

Glycosyltransferase cascade: Fusion with the binding module Zbasic2 enables facile, one-step purification-immobilization of sialic acid synthetase and ?2,3-sialyltransferase on anionic carrier. The immobilized enzymes are recyclable catalysts for one-pot cascade synthesis sialo-oligosaccharides.

In this paper glucoamylase from Aspergillus niger was immobilized by using a modified version of cross-linked enzyme aggregates (CLEA). The co-aggregates were cross-linked with glutaraldehyde; meanwhile dextrin and xanthan gum as protecting agents were added, which provides high affinity with the enzyme molecules. The immobilized glucoamylase was stable over a broad range of pH (3.0–8.0) and te...

Glucose oxidase, uricase, and urease were immobilized on the interior surface of activated polyamide tubing. The shelf-life of such enzyme bearing tubes was at least six months. The tubes were used for continuous-flow analysis of glucose, uric acid, and urea with conventional systems and with hybrid micro-scale systems in which modules of different manufacture were combined. The length of enzym...

Abstract Enzymes immobilized onto substrates with excellent selectivity and activity show a high stability can withstand extreme experimental conditions, their performance has been shown to be retained after repeated uses. Applications of enzymes in various fields benefit from unique characteristics. Common methods, including adsorption, encapsulation, covalent attachment crosslinking, other em...

Sugar coatings can stabilize the "native" structure and control the orientation of surface-immobilized peptides in air, providing a potential approach to retain biological functions of surface-immobilized biomolecules in air. This method is general and applicable to complex enzymes.