During the last ten years the extraction of specific antibodies (ab) from the yolk of eggs of immunised chickens is more and more accepted as an useful alternative to the immunisation of mammals. The subject of this work is the immunisation of chickens with human IgG and the extraction of specific anti human IgG ab from egg yolk in order to obtain monospecific Coombs reagent. 12 Leghorn hens (2...

background helicobacter pylori multiplies and causes infection in human gastric mucosal layer. new approaches have focused on using specific treatments, such as immunotherapy, to limit this infection. urease, as one of the most important virulent and antigenic factors of the bacterium, is a suitable target for this purpose. patients and methods in order to prepare recombinant proteins, the synt...

West Nile virus (WNV) causes morbidity and mortality in humans, horses, and in more than 315 bird species in North America. Currently approved WNV vaccines are designed for parenteral administration and, as yet, no effectiveoral WNV vaccines have been developed. WNV envelope (E) protein is a highly antigenic protein that elicits the majority ofvirus-neutralizing antibodies during a WNV immune r...

We investigated the effects of ecological context (by comparing data from two consecutive years) and experimentally manipulated nestling developmental conditions (large vs. small brood size) on immune function (immunoglobulin Y [IgY]) and oxidative stress in nestling European starlings Sturnus vulgaris. On the basis of annual differences in chicks' morphological traits and body masses close to ...

Avian coccidiosis is caused by several distinct species of Eimeria protozoa and is the major parasitic disease of poultry of economic importance. As an alternative strategy to control avian coccidiosis without using prophylactic medications, we have investigated the efficacy of inducing passive immunity against coccidiosis by orally feeding hyperimmune IgY antibodies. In this study, a commercia...

In order to improve the sensitivity of proteomic analysis, our group has developed a chicken antibody based column that has successfully depleted high abundance proteins (HAPs) from human plasma prior to mass spectrometry analysis. The use of chicken as a host for polyclonal antibodies production conveys many advantages, including but not limited to, the capabilities of producing large quantiti...

The bacteria Pasteuria spp. have been identified as among the most promising of several microbial organisms currently under investigation as biological control agents of plant-parasitic nematodes. As part of our goal to develop methods to discriminate isolates of Pasteuria penetrans with different host preferences, we investigated the potential of developing antibody probes to identify endospor...

Previous studies on the immunoglobulin (Ig) genes in avian species are limited (mainly to galliformes and anseriformes) but have revealed several interesting features, including the absence of the IgD and Igκ encoding genes, inversion of the IgA encoding gene and the use of gene conversion as the primary mechanism to generate an antibody repertoire. To better understand the Ig genes and their e...

Previous studies on a limited number of birds suggested that the IgD-encoding gene was absent in birds. However, one of our recent studies showed that the gene was definitely expressed in the ostrich and emu. Interestingly, we also identified subclass diversification of IgM and IgY in these two birds. To better understand immunoglobulin genes in birds, in this study, we analyzed the immunoglobu...

“Researchers would like to thank the Deanship of Scientific Research, Qassim University for funding publication this project”.“This work was supported by a grant from University” Anti-E. coli Immunoglobulin Yolk (IgY): Reduction pathogen receptors and inflammation factors could be caused decrease in E. loadBouazzaoui et al.HeliyonFebruary 21, 2023In BriefGraft versus host disease (GVHD) remains...