Preparative HPLC 5}30 '20 2}20 0.01}1 High purity solvent required Higher resolution See also: II/Chromatography: Countercurrent Chromatography and High-Speed Countercurrent Chromatography: Instrumentation. Chromatography: Liquid: Countercurrent Liquid Chromatography. III/Alkaloids: Gas Chromatography; High-Speed Countercurrent Chromatography; Liquid Chromatography; Thin-Layer (Planar) Chromato...

A sensitive (0.01-1 nmol) method has been developed for the analysis of polyamines in higher plant extracts based on high performance liquid chromatography (HPLC) of their benzoyl derivatives (Redmond, Tseng 1979 J Chromatogr 170: 479-481). Putrescine, cadaverine, agmatine, spermidine, spermine, and the less common polyamines nor-spermidine and homospermidine can be completely resolved by rever...

Derris trifoliata belongs to the family Fabaceae of the order Papilionoideae. D. trifoliata is used in local medicine in India as a stimulant, antispasmodic and counter-irritant, and against rheumatism, chronic paralysis and dysmenorrhoea. The bioactive principle present in the leaves of Derris trifoliata was isolated and characterized in the present study. The powdered leaves were repeatedly e...

A mutant of Mycobacterium smegmatis defective in mycolic acid biosynthesis was isolated following chemical mutagenesis. Fatty acids were extracted from the mutant and subjected to structural analysis by thin-layer chromatography and high-performance liquid chromatography (HPLC) of both methyl and p-bromophenacyl ester derivatives. Thin-layer chromatography did not show the presence of any fatty...

application of atoxigenic strains of aspergillus flavus to reduce aflatoxin levels is the most successful strategy applied in some agricultural crops. the role of ammonium hydroxide for preliminary screening of the competitiveness of atoxigenic a. flavus isolates to interfere with aflatoxin production by highly toxigenic isolates were evaluated. out of 270 a. flavus isolates, 17 were detected a...

The newest achievements in the application of miscellaneous liquid chromatographic techniques such as size-exclusion, ion-exchange and reversed-phase high-performance liquid chromatography, and thin-layer chromatography for the elucidation of the various aspects of the binding of ligands to proteins are compiled and briefly discussed. Examples of employment in pharmaceutical and clinical chemis...

Chlorophylis cl and c2 have been separted from total pigment extracts of the alg Pavlovagyras Butcher using a reversed-phae high-performance liquid chromatography system. Pigments were separated on a 5 micrometer Cl, column (25 centimeters x 4.6 millimeters) using a gadient ofmethanol-acetonitrile-water. Other photosynthetic pigments were also well resolved by the system used. The separation sy...

In the last decade we have been working on standardization of propolis extract and determination of active constituents of wine those are rich in polyphenolics and have nutritional as well as therapeutic value. Here we are summarizing our results and providing overview on systematic approach how to analyse natural products rich in flavonoids and phenolic acids.Chromatographic methods (thin laye...

TLC screening and Evaluation of antioxidant, antibacterial activity of Onopordon macrocephalum by bioautography methodOnopordon known as scotch thistle is a medicinal plant of the Asteraceae family that is widely distributed in Europe and Asia, also it has many valuable medicinal properties as hypotensive and antitumor. Thin layer chromatography (TLC) is wildly used in natural product ext...