The Treponema pallidum haemagglutination (TPHA) test was carried out on 274 sera known to show biological false positive reactions to reagin tests for syphilis. The Treponema pallidum immunization (TPI) and fluorescent treponemal antibody absorption (FTA-ABS) tests were non-reactive on all these sera. Thirty-one or 11.3% showed reactive results in the TPHA test. Sera from 267 people who had lep...

The specificity and sensitivity of two commercial enzyme linked immunosorbent assays (ELISAs), Diamedix (Miami, Florida) and Lipogen (Noxville, Tennessee), were assessed and compared with haemagglutination and immunodiffusion assays. Sera from 53 patients with various connective tissue disorders were examined for the presence of antibodies to nuclear antigens (ANA), double stranded DNA (dsDNA),...

The present study was designed to investigate effects of Lisovit (Biomin, Austria) on the immune system of broiler chickens. For this purpose, 270 (day-old) broiler chicks were randomly divided into three groups A, B and C of 90 birds each. The birds were vaccinated against Newcastle Disease (ND) on day 3 (intraocular) and day 18 (drinking water) of age and against Infectious Bursal Disease (IB...

Using a modified cell-free culture medium, a mycoplasma was isolated from sarcoid lymph nodes in two cases and from sarcoid skin lesions in four out of seven cases of chronic sarcoidosis. Growth inhibition tests showed that the isolates were related to Mycoplasma orale type 1. By the indirect haemagglutination method, 244 cases of definite or probable sarcoidosis, 160 patients with other diseas...

Between January 1990 and September 1992, thirty patients ranging in age from 7 months to 43 years with a diagnosis of dengue fever (DF) or dengue haemorrhagic fever (DHF) were admitted to the Intensive Care Unit (ICU) of the University Hospital, Kuala Lumpur. 21 of these patients were serologically confirmed to have dengue infection by either IgM-capture ELISA andfor haemagglutination inhibitio...

The treponemal haemagglutination (THA) test has been used for 11 years as a routine test for syphilis at a central serology laboratory. Among patients with untreated early syphilis the antibodies detected by the THA test usually appeared later or were of relatively lower titre than those detected by the reagin tests. This finding was confirmed by follow up of patients and by replies to question...

To study the neutralizing epitopes of influenza B virus Yamagata group strains, two monoclonal antibodies (mAbs) were used to select escape mutants of the virus. mAbs 5H4 and 3A12 were found to react with B/Yamagata group strains in haemagglutination inhibition and neutralization tests; no reactivity with B/Victoria group strains was observed. Most of the mutants reacted poorly to polyclonal fe...

A 15-year-old girl, who was evaluated for arthralgia of knees, was diagnosed as having brucellosis by serum agglutination and enzyme linked immunosorbent assay tests. Physical examination of the patient revealed massive hepatomegaly. Abdominal ultrasonography and computerised tomography showed a single large cystic lesion of the liver. The echinococcus indirect haemagglutination was positive at...