Glycosylphosphatidylinositol (GPI) protein anchors are ubiquitous in eukaryotic cells. GPI anchors are synthesized in the endoplasmic reticulum by actions of ten or more gene products. The first step of the biosynthesis, the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, is mediated by at least three genes in mammalian cells (PIG-A, PIG-H and PIG-C) and in...

A radioimmunoassay for the determination of pig pancreatic kallikrein was developed. The chloramine-T method was employed for the labelling of the antigen with 125I. The assay allows the determination of kallikrein in concentrations as low as 0.4 microgram/l. Pig urinary and pig submandibular kallikreins are indistinguishable from pig pancreatic kallikrein by the assay. No cross reactivity was ...

The fertility results of pig artificial insemination (AI) in The Netherlands are considered to be one of the highest worldwide. More than 98% of the sows in The Netherlands is mated through AI. Sow farmers put great trust in AI and are fully depending on the quality of the product. The Dutch cooperative pig AI centers put large efforts in producing high quality insemination doses as far as seme...

The aim of this field study was to determine the concentrations and emissions of ammonia and hydrogen sulfide in different types of pig buildings in Korea to allow objective comparison between pig housing types in Korea and other countries. Concentrations of ammonia and hydrogen sulfide in the pig buildings averaged 7.5ppm and 286.5ppb and ranged from 0.8 to 21.4ppm and from 45.8 to 1235ppb, re...

Pig phospholipase A2, pig iso-phospholipase A2 and bovine pancreatic phospholipase A2 were reacted in solution with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, in the presence of N-hydroxysulfosuccinimide, at pH 7. The influence of micellar protectants was analyzed. In the presence of n-hexadecylphosphocholine, the losses of activity in micellar diheptanoyl-lecithin were 80, 35, and 10% in b...

To study knowledge, attitude and risk factors of Japanese Encephalitis (JE), a research was conducted from May to November 2012 in Rupandehi district Nepal including household (HH) survey, pig survey and swine sero survey. Questionnaire survey on One hundred HH (50 pig raisers and 50 pig non raisers) to compare JE risk factors; 100 pig farmers to study roles of pig as risk factor for JE in huma...

Escherichia coli F18 bacteria producing enterotoxins and/or shigatoxin (ETEC/STEC) are main pathogens that cause oedema disease and postweaning diarrhoea in piglets, and alpha-1-fucosyltransferase (FUT1) gene has been identified as a candidate gene for controlling the expression of ETEC F18 receptor. The genetic variations at nucleotide position 307 in open reading frame of FUT1 gene in one wil...

background in recent decades, bacterial antibiotic resistance (especially in enterococci) has become a significant problem for human and veterinary medicine. one of the most important antibiotic resistances in enterococci, vancomycin resistance, is encoded by van gene family. objectives the aim of this study was to investigate antibiotic resistance to vancomycin in enterococci and the genes res...

High density lipoproteins (HDL) were isolated from pig plasma by ultracentrifugal flotation between densities 1.063 and 1.210 g per ml. After delipidation, the apolipoproteins were fractionated by chromatography on Sephadex G-150 and DEAE-cellulose in 5.4 M urea. One major apolipoprotein was isolated and characterized. From its chemical and physical properties, this major apolipoprotein appears...

The information given to consumers is essential for them to choose one food product over another. The falsification of food contents on product labels is a widespread problem, especially with products related with pig or others prohibited food in Islam. Proving conclusively that fraud has occurred requires the detection and quantification of food constituents. Falsifications of meat or food are...