Important progress in understanding replication, interactions with host plants, and evolution of closteroviruses enabled engineering of several vectors for gene expression and virus-induced gene silencing. Due to the broad host range of closteroviruses, these vectors expanded vector applicability to include important woody plants such as citrus and grapevine. Furthermore, large closterovirus ge...

Background: Development of an effective vaccine is highly needed in order to restrict the AIDS pandemic. DNA vaccines initiate both arms of immunity without the potential of causing disease. HIV-1 p24 and gp41 (gag and env) proteins play important roles in viral pathogenesis and are effective candidates for immune induction and vaccine design. Objective: In this study, new DNA vaccine candidate...

We seek to create useful biological diversity by exploiting the modular nature of genetic information. In this report we describe experiments that focus on the modular nature of plasmid cloning vectors. Bacterial plasmids are modular entities composed of origins of replication, selectable markers and other components. We describe a new ligation-independent cloning method that allows for rapid a...

In the past, many researchers considered viral vectors to be the most promising candidates to transfer genetic material into the corpora for the treatment of erectile dysfunction. However, at present, no viral vectors have progressed to human trials. In contrast, the use of naked gene therapy, a plasmid expressing the human Maxi-K potassium channel, is the only gene therapy treatment to be eval...

The ability to repair or enhance an individual's genetic make-up provides the sublime opportunity to ameliorate or eliminate many clinical disorders that affect mankind. Gene therapy is thus a revolutionary clinical strategy that may potentially treat an array of genetic and non-genetic diseases, as well as a novel method for drug delivery and vaccination. To these ends, adenovirus vectors are ...

introduction: cutaneous leishmaniasis is a parasitological disease that is spread in many tropical and subtropical areas in world. this disease transmitted by female sand flies. it is clear that knowledge on sand flies ecology has the main role to planning of control of cl. this study was carried out for determination of cl vectors, in ghanavat district in qom province during 2012. material and...

As of May 2001, 532 gene therapy protocols had been approved for evaluation in clinical trials; however, only five of those had been evaluated in phase III clinical trials. Among the most commonly used vectors for the delivery of genetic material into human cells are the adenoviruses. Remarkable progress has been made with these vectors in the last decade, but some shortcomings continue to chal...

In this paper, a hierarchical genetic algorithm for disparity estimation is presented. The goal, to estimate reliable disparity fields with low computational cost, is reached using a hierarchical genetic matching procedure. Firstly, each hierarchical image of the stereo pair is divided into sets of feature points and non-feature points. The image morphological gradient for feature points and th...

Ritz method is one of the techniques for reduction of the degrees of freedom. Efficiency of Ritz method depends on used vectors. The Ritz method uses load depended vectors in spite of modal method and for this reason it is expected to give better results than modal method. This is the advantage of Ritz method. It is worth mention that the mode shapes are independent of loading. The vectors that...

Protein fold classification is a key step to predicting protein tertiary structures. This paper proposes a novel approach based on genetic algorithms and feature selection to classifying protein folds. Our dataset is divided into a training dataset and a test dataset. Each individual for the genetic algorithms represents a selection function of the feature vectors of the training dataset. A sup...