The control of the three-dimensional (3D) polymer network structure is important for permselective materials when specific biomolecule detection needed. Here we investigate conditions to obtain a tailored hydrogel that combines both molecular filtering and capture capabilities biosensing applications. Along this line, short oligonucleotide in displacement assay set within PEGDA hydrogels synthe...

in the present study, an oxidative conversion of non-fluorescent dichlorofluoroscein (ldcf) to fluorescent dichlorofluoroscein (dcf) was used for detection of antioxidant properties of plant extracts. the rate of the reaction was followed by monitoring the formation of dcf as a function of time. the antioxidant assay was carried out for different concentrations of gallic acid, salvia mirzayanii...

Oxyresveratrol (OXY) is a small molecule phytochemical which has been reported to have important biological function. The aim of this study was elucidate the gene expression and pathways altered in MCF-7, breast cancer cells following exposure OXY. cytotoxicity different cell lines screened using MTT assay then whole elucidated microarray. selected were also validated by quantitative PCR analys...

We investigated measuring serum alpha-L-iduronidase (EC 3.2.1.76) by a sensitive fluorometric assay in 28 members of a canine family with mucopolysaccharidosis I. If assayed on the day of collection, serum was an acceptable specimen for identifying affected, carrier, and normal dogs. The overall correlation (r) between iduronidase activity in serum and mononuclear leukocytes was 0.966. However,...

The effect of the administration of a synthetic peptide with corticotrophic properties (18-ACTH) on the plasma corticosteroid levels of 72 healthy volunteers was studied. The same effect is seen both after subcutaneous and intramuscular injection. Intravenous administration provokes a longer lasting influence on adrenocortical activity, especially when large doses of the peptide are given; side...

An in vivo-in vitro alkaline DNA unwinding assay for single-strand breaks and/or alkali-labile lesions in hepatic DNA is described. The assay involves isolation of hepatic nuclei from mice, alkaline denaturation and unwinding of hepatic DNA, separation of single- and double-stranded DNA by hydroxylapatite batch chromatography, and quantitation of DNA in chromatographic fractions by a fluorometr...