Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determ...

The aim of the study has been to estimate the effect of culture conditions and a culture site on magnesium (Mg) concentrations in freshwater fish. The study encompassed tissues (blood) and organs (gills, liver, kidney, dorsal muscles) of five fish species: common carp (Cyprinus carpio L.), rainbow trout (Oncorhynchus mykiss Walbaum), Siberian sturgeon (Acipenser baeri Brandt), northern pike (Es...

Increased production of fish through intense culture practices often leads to not only stress and disease problems but also alteration in water quality. The organic wastes and bad water quality influence the growth of pathogenic microorganisms. Bioremediation using pond probiotics is considered a promising way to circumvent these problems. For this study, three earthen fish ponds stocked with P...

The study designed and constructed a prototype recirculating aquaculture system (RAS) which was used to examine the liver and kidney histology of hybrid catfish (Heterobranchus bidorsalis (♂) X Clarias gariepinus (♀)) fed six practical diets after 12 weeks. Fingerlings of the test fish, mean weight and length of 1.01±0.02g and 4.63±0.34cm were used for the study. In the formulated diets fishmea...

Introduction Atypical Aeromonas salmonicida is a fish pathogen that causes several diseases in freshwater fish such as goldfish (Carassius auratus L.) (6) and eel (Anguilla japonica) (2), and also seawater fish (5). Since 1996, A. salmonicida infection characterized by the formation of ulcers on the body surface and fins (ulcer disease) has become prevalent in colored carp (Cyprinus carpio koi ...

Viral hemorrhagic septicemia virus (VHSV) infects a wide range of marine fish species. To study the occurrence of VHSV in wild marine fish populations in Norwegian coastal waters and fjord systems a total of 1927 fish from 39 different species were sampled through 5 research cruises conducted in 2009 to 2011. In total, VHSV was detected by rRT-PCR in twelve samples originating from Atlantic her...

The molecular properties and roles of luteinizing hormone (Lh) and its receptor (Lhcgrbb) have not been studied for the medaka (Oryzias latipes), which is an excellent animal model for ovulation studies. Here, we characterized the medaka Lh/Lhcgrbb system, with attention to its involvement in the ovulatory process of this teleost fish. In the medaka ovary, follicle-stimulating hormone receptor ...

Environmental microbiology utilizes indirect methods as microscopy and culture media to its studies. However, these conventional methodologies present problems to quantify and qualify microorganisms and their relation with the environment. The aim of this manuscript was to describe the application of fluorescent in situ hybridization (FISH) in environmental microbiology studies. It was discusse...

A nested reverse transcriptase (RT) PCR assay detected mRNA of the salmonid pathogen Renibacterium salmoninarum in samples of RNA extracts of between 1 and 10 cells. Total RNA was extracted from cultured bacteria, Atlantic salmon (Salmo salar L.) kidney tissue and ovarian fluid seeded with the pathogen, and kidney tissue from both experimentally challenged and commercially raised fish. Followin...