Nowadays, the most rapid method for producing healthy and disease-free Lisiantus is micropropagation. With respect to high economic value of this plant which is regarded among the 10 top cutting flowers in the world, this research was carried out to suggest a suitable protocol for its in vitro propagation, using nodal sections as an explant. To carry out this object, the effects of the pH (5.5,...

AIMS Cultured cardiac explants produce a heterogeneous population of cells including a distinctive population of refractile cells described here as small round cardiac explant derived cells (EDCs). The aim of this study was to explore the source, morphology and cardiogenic potential of EDCs. METHODS Transgenic MLC2v-Cre/ZEG, and actin-eGFP mice were used for lineage-tracing of EDCs in vitro a...

in order to study, in vitro bud induction and shoot regeneration of different explants (shoot tip, axillary bud, hypocotyl and cotyledon) of henbane on ms medium enriched with various concentrations of kinetin (0, 1, 3 and 5 mg.l-1) alone or in combination with indole-3 acetic acid (0, 0.1 and 0.5 mg.l-1) this research was investigated in urom zist tak knowledge-based company plant tissue cultu...

Attempts were made to superinfect epithelial explant cell cultures prepared from nasopharyngeal carcinomas with Epstein-Barr virus. Virus-specific markers were observed in such cultures 3 days after superinfection. In addition, expression of Epstein-Barr virus early antigens was observed in epithelial cell explant cultures treated with iododeoxyuridine. The results suggest that epithelial cells...

Development of a micropropagation system for a new species or cultivar can be a daunting task and hundreds of decisions are involved in even deciding where to start. Dozens of basal media, growth regulators, and supplemental nutrients are available for use. Environmental conditions to be considered include light intensity and quality, photoperiod, temperature, container, amount of medium, liqui...

The complex process of axon guidance is largely driven by the growth cone, which is the dynamic motile structure at the tip of the growing axon. During axon outgrowth, the growth cone must integrate multiple sources of guidance cue information to modulate its cytoskeleton in order to propel the growth cone forward and accurately navigate to find its specific targets(1). How this integration occ...