aims: in our previous studies, we showed the inhibitory effects of punica granatum l. flower and rhus coriaria l. fruit water extracts on dental plaque accumulation by several bacteria, especially streptococcus mutans (s. mutans), on orthodontic wire by in vitro assays. in this study, the anti-cariogenic properties of the extracts were evaluated by assessing their effects on expression of glyco...

Aims: In our previous studies, we showed the inhibitory effects of Punica granatum L. flower and Rhus coriaria L. fruit water extracts on dental plaque accumulation by several bacteria, especially Streptococcus mutans (S. mutans), on orthodontic wire by in vitro assays. In this study, the anti-cariogenic properties of the extracts were evaluated by assessing their effects on expression of glyco...

The development biofuels from Lignocellulosic (LC) biomass, which includes a variety of plant feedstocks such as agricultural and forestry residues and dedicated energy crops, is one route to clean and renewable alternatives to petroleum-base fuel. The first step in converting plant biomass to fuels is breaking down cellulose, the most abundant plant cell wall polymer, into fermentable sugars. ...

Members of the SNF2 family of ATPases often function as components of multi-subunit chromatin remodeling complexes that regulate nucleosome dynamics and DNA accessibility by catalyzing ATP-dependent nucleosome remodeling. Biochemically dissecting the contributions of individual subunits of such complexes to the multi-step ATP-dependent chromatin remodeling reaction requires the use of assays th...

Carbohydrate active enzymes often contain auxiliary binding sites located either on independent domains termed carbohydrate binding modules (CBMs) or as so-called surface binding sites (SBSs) on the catalytic module at a certain distance from the active site. The SBSs are usually critical for the activity of their cognate enzyme, though they are not readily detected in the sequence of a protein...

glutathione s-transferases (gsts) are widespread in fasciola. hepatica parasite and sheep liver tissue. study of gsts inhibition assays in f. hepatica and sheep liver tissue are a priority of chemotherapeutic targets in parasitic liver diseases including human fascioliasis in iran. in this research, the whole extract of f. hepatica and sheep liver tissues were purified and eluted for sodium dod...

In this study we describe a battery of fluorescence assays for rapid measurement in intact cells of the activity of nine cytochromes P450 (P450s) involved in drug metabolism. The assays are based on the direct incubation of monolayers of cells expressing individual P450 enzymes with a fluorogenic substrate followed by fluorimetric quantification of the product formed and released into incubatio...